The surface-exposed carboxyl region of Mycoplasma pneumoniae elongation factor Tu interacts with fibronectin.

Infection and Immunity
Sowmya BalasubramanianJ B Baseman

Abstract

Mycoplasma pneumoniae is a bacterial pathogen of the human respiratory tract that causes a wide range of airway diseases as well as extrapulmonary symptoms. It possesses a distinct, differentiated terminal structure, termed the attachment organelle, that mediates adherence to the host respiratory epithelium. Previously, we reported that surface-associated M. pneumoniae elongation factor Tu (EF-Tu, also called MPN665) serves as a fibronectin (Fn)-binding protein, facilitating interactions between mycoplasmas and extracellular matrix. In the present study, we determined that binding of M. pneumoniae EF-Tu to Fn is primarily mediated by the EF-Tu carboxyl region. A 179-amino-acid region spanning the carboxyl terminus (designated EC; amino acids 192 to 394) binds Fn in a dose-dependent manner. Further analysis of carboxyl constructs (ED3 and ED4) and their deletion truncations (ED3.1, ED3.2, and ED4.1) revealed that the carboxyl region possessed two distinct sites with different Fn-binding efficiencies. Immunogold electron microscopy using antibodies raised against recombinant ED3 and ED4 demonstrated the surface accessibility of the EF-Tu carboxyl region. Competitive binding assays using intact radiolabeled mycoplasmas and purifie...Continue Reading

References

Mar 1, 1979·Infection and Immunity·E J HansenJ B Baseman
May 6, 1976·Nature·G R Jacobson, J P Rosenbusch
May 1, 1977·The Journal of Experimental Medicine·P C HuJ B Baseman
Jul 1, 1992·European Journal of Biochemistry·S BozziniP Speziale
May 1, 1991·Journal of Bacteriology·C C Young, R W Bernlohr
Dec 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·A M CollierF W Denny
Nov 19, 1980·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·T Blumenthal
Apr 1, 1981·Infection and Immunity·E J HansenJ B Baseman
Jun 1, 1983·The Journal of Experimental Medicine·K M PetersonJ F Alderete
Aug 1, 1984·Infection and Immunity·P KuuselaE B Myhre
Sep 1, 1982·Journal of Bacteriology·J B BasemanD K Leith
Jan 1, 1994·Annual Review of Microbiology·J M PattiM Höök
Jan 1, 1996·Advances in Experimental Medicine and Biology·E HanskiV Ozeri
Jun 13, 1998·The Journal of Biological Chemistry·T D CaldasG Richarme
Oct 24, 1998·Cell Motility and the Cytoskeleton·R C MooreR J Cyr
Nov 14, 1998·Biochemical and Biophysical Research Communications·G Richarme
Dec 5, 1998·Biochimica Et Biophysica Acta·I M Krab, A Parmeggiani
Aug 3, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·D JohM Höök
Oct 14, 2000·Microbial Pathogenesis·S F Dallo, J B Baseman
Mar 29, 2001·Electrophoresis·J T RegulaR Frank
Jul 11, 2002·Annals of Medicine·Harry S CourtneyJames B Dale
May 22, 2003·Cell Biology International·F Mayer
Jun 6, 2003·Molecular Microbiology·René A AlvarezJoel B Baseman
Mar 9, 2004·Journal of Molecular Biology·Grégory BoëlJosef Deutscher
Apr 23, 2004·Molecular Microbiology·Ulrich Schwarz-LinekJennifer R Potts
Sep 21, 2004·Proteomics·Jessica SchaumburgUwe Kärst
Aug 11, 2005·Molecular Microbiology·Michael E KonkelSteve G Garvis

❮ Previous
Next ❯

Citations

Jan 20, 2011·The Journal of Biological Chemistry·Lisa M SeymourMark J Walker
Mar 9, 2012·The Journal of Biological Chemistry·Keisuke ItagakiFumio Fukai
Oct 1, 2011·The Journal of Infectious Diseases·T R KannanJoel B Baseman
Jan 1, 2012·ISRN Veterinary Science·Manuel Sebastián Rebollo CoutoHernán Terenzi
Aug 20, 2014·BioMed Research International·Mariana Noelia VialeMaría de la Paz Santangelo
Aug 26, 2014·Journal of Proteomics·Caroline Le MaréchalGwénaël Jan
Feb 3, 2009·Microbial Pathogenesis·Katsushiro MiyamotoHiroshi Tsujibo
Jul 22, 2008·Vaccine·Roy RobertsDaniela Hozbor
Sep 1, 2015·FEMS Microbiology Letters·Shijun BaoChan Ding
Oct 7, 2014·Research in Microbiology·Laura CastroAntonio Ballester
Aug 11, 2010·FEMS Microbiology Reviews·Brian HendersonMark A Williams
Aug 8, 2013·Virulence·G Brett Moreau, Barbara J Mann
Sep 13, 2016·International Journal of Medical Microbiology : IJMM·Anne GründelRoger Dumke
Aug 10, 2017·International Journal of Molecular Sciences·Yongpeng GuoXi Chen
Jan 1, 2015·Emerging Microbes & Infections·Sebastian E CarrascoX Frank Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.