Abstract
It is well known that the Na+ cycle and the cell wall are essential for alkaline adaptation of Na+-dependent alkaliphilic Bacillus species. In Bacillus pseudofirmus OF4, surface layer protein A (SlpA), the most abundant protein in the surface layer (S-layer) of the cell wall, is involved in alkaline adaptation, especially under low Na+ concentrations. The presence of a large number of genes that encode S-layer homology (SLH) domain-containing proteins has been suggested from the genome sequence of B. pseudofirmus OF4. However, other than SlpA, the functions of SLH domain-containing proteins are not well known. Therefore, a deletion mutant of the csaB gene, required for the retention of SLH domain-containing proteins on the cell wall, was constructed to investigate its physiological properties. The csaB mutant strain of B. pseudofirmus OF4 had a chained morphology and alkaline sensitivity even under a 230 mM Na+ concentration at which there is no growth difference between the parental strain and the slpA mutant strain. Ultra-thin section transmission electron microscopy showed that a csaB mutant strain lacked an S-layer part, and its peptidoglycan (PG) layer was disturbed. The slpA mutant strain also lacked an S-layer part, alth...Continue Reading
References
Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Jul 1, 1992·The Biochemical Journal·R AonoK Horikoshi
Nov 1, 1987·Analytical Biochemistry·H Schägger, G von Jagow
Mar 1, 1989·Journal of Bacteriology·S ClejanT A Krulwich
Feb 1, 1984·Journal of Bacteriology·R AonoS Goto
Feb 1, 1995·Molecular Microbiology·T A Krulwich
Jun 1, 1997·Journal of Bacteriology·M ItoT A Krulwich
Oct 28, 1999·Microbiology·H MorisakiK Kogure
Nov 5, 1999·Journal of Bacteriology·R AonoT Machida
Sep 6, 2000·The EMBO Journal·S MesnageA Fouet
Oct 13, 2000·Journal of Bacteriology·R GilmourT A Krulwich
Mar 15, 2001·Biochimica Et Biophysica Acta·T A KrulwichA A Guffanti
Feb 28, 2002·Bioscience, Biotechnology, and Biochemistry·Masahiro Ito, Rikizo Aono
Jun 19, 2003·Journal of Bacteriology·Shujiro OkudaHisao Morisaki
Jul 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Masahiro ItoTerry A Krulwich
Aug 13, 2004·Molecular Microbiology·Masahiro ItoTerry A Krulwich
Mar 11, 2005·Microbiology·Christina Schäffer, Paul Messner
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kittichoat TiyanontSuzanne Walker
Jul 21, 2006·Journal of Bacteriology·Tatsuya FukushimaJunichi Sekiguchi
Dec 14, 2006·Archives of Microbiology·Shun FujinamiMasahiro Ito
Dec 1, 2007·Microbiology·Shun FujinamiMasahiro Ito
Jul 7, 2010·Journal of Molecular Biology·Justin KernOlaf Schneewind
Jul 29, 2010·Environmental Technology·Shun Fujinami, Makoto Fujisawa
May 19, 2011·Molecular Microbiology·Valerie J AndersonDominique Missiakas
Jul 26, 2011·Extremophiles : Life Under Extreme Conditions·Shun FujinamiMasahiro Ito
Sep 29, 2011·Environmental Microbiology·Benjamin JantoTerry A Krulwich
Dec 18, 2012·Journal of Bacteriology·J Mark LunderbergOlaf Schneewind
Oct 22, 2013·Journal of Bacteriology·Masato MorinoTerry Ann Krulwich