PMID: 179591May 21, 1976Paper

The surface membrane of the small intestinal epithelial cell. I. Localization of adenyl cyclase

Biochimica Et Biophysica Acta
H MurerU Hopfer

Abstract

The subcellular distribution of adenyl cyclase was investigated in small intestinal epithelial cells. Enterocytes were isolated, disrupted and the resulting membranes fractionated by differential and sucrose gradient centrifugation. Separation of luminal (brush border) and contra-luminal (basolateral) plasma membrane was achieved on a discontinuous sucrose gradient. The activity of adenyl cyclase was followed during fractionation in relation to other enzymes, notably those considered as markers for luminal and contraluminal plasma membrane. The luminal membrane was identified by the membrane-bound enzymes sucrase and alkaline phosphatase and the basolateral region by (Na+ + K+)-ATPase. Enrichment of the former two enzymes in purified luminal plasma membrane was 8-fold over cells and that of (Na+ + K+)-ATPase in purified bisolateral plasma membranes was 13-fold. F--activated adenyl cyclase co-purified with (Na+ + K+)-ATPase, suggesting a common localization on the plasma membrane. The distribution of K+-stimulated phosphatase and 5'-nucleotidase also followed (Na+ + K+)-ATPase during fractionation.

References

Jan 1, 1975·Annual Review of Physiology·I M Glynn, S J Karlish
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S H OngA L Steiner
Feb 1, 1973·The Journal of Cell Biology·J W DePierre, M L Karnovsky
Jan 22, 1971·Nature·G W Sharp, S Hynie
Jun 1, 1971·The Journal of Clinical Investigation·D V KimbergE Gershon
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·D E SchaferN D Goldberg
Sep 1, 1972·The Journal of Clinical Investigation·D K ParkinsonG W Sharp
Jul 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·I L SchwartzR Kinne
Jun 1, 1972·The Journal of Cell Biology·C E Stirling
Jul 18, 2006·Journal of the American Veterinary Medical Association·Carley A SaelingerCarol L Lemons

Citations

Nov 22, 2001·Biochemical and Biophysical Research Communications·P MunroP Boquet
Nov 1, 1989·Pflügers Archiv : European journal of physiology·D W WatkinsP Ripoche
Apr 1, 1992·Pflügers Archiv : European journal of physiology·A De GiorgiA Corcelli
Mar 20, 1978·Pflügers Archiv : European journal of physiology·H LúckeH Murer
Nov 11, 1982·Pflügers Archiv : European journal of physiology·Giulia GastaldiG Rindi
Aug 1, 1986·Biulleten' eksperimental'noĭ biologii i meditsiny·A P KhokhlovA G Shitin
Apr 1, 1989·Digestive Diseases and Sciences·A MoritaY S Kim
Nov 30, 1979·The Journal of Membrane Biology·A K MircheffE M Wright
Jul 15, 1980·The Journal of Membrane Biology·H Murer, R Kinne
Nov 1, 1988·The Journal of Membrane Biology·M C LinF A Wilson
Apr 15, 1981·The Journal of Membrane Biology·D J Culp, J G Forte
Jan 1, 1994·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·J SteinW F Caspary
Sep 12, 2008·Pharmaceutical Research·Takanori HironakaToshikiro Kimura
Jan 1, 1981·The International Journal of Biochemistry·K Y LingR G Faust
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·A J MoeM J Jackson
Sep 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·B WindingN Bindslev
Sep 23, 1997·Mechanisms of Ageing and Development·I PohlováJirí Pácha
Feb 22, 2002·European Journal of Biochemistry·Sylvie Smialowski-FléterA Puigserver
Mar 23, 2000·European Journal of Biochemistry·S BerardiS Krähenbühl
May 3, 2000·Kidney International·Giulia GastaldiG Rindi
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P DomínguezP S Lazo
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J ZhouJ Douglas
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E P BurkeB Horowitz
Sep 1, 1980·The Journal of Cell Biology·C A ZiomekM Edidin
May 28, 2008·The Journal of General Physiology·Gustavo FrindtLawrence G Palmer
Feb 1, 1985·European Journal of Biochemistry·P DomínguezP S Lazo
Oct 30, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Chengbo YangMing Z Fan
Apr 30, 1985·Biochemical and Biophysical Research Communications·P Q BarrettH Rasmussen
Jun 1, 1985·Archives of Biochemistry and Biophysics·P S LazoG Velasco
Oct 16, 1999·Biochimica Et Biophysica Acta·F Dalle OreA Puigserver
Dec 1, 1983·Cancer Letters·N J Temple, S M El-Khatib
Mar 16, 1990·Biochemical and Biophysical Research Communications·J M HarigT A Brasitus
Apr 4, 2009·Biochemical and Biophysical Research Communications·Graça SoveralTeresa F Moura
Jan 10, 2002·Biochimica Et Biophysica Acta·Anna VerriGianguido Rindi
Jun 27, 1984·Biochimica Et Biophysica Acta·T A Brasitus, R S Keresztes
Feb 9, 1983·Biochimica Et Biophysica Acta·T A Brasitus, R S Keresztes
Aug 21, 2002·Journal of Pediatric Gastroenterology and Nutrition·Yuriko Adkins, Bo Lönnerdal
Feb 17, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ming YuRichard J Roman
Aug 17, 2002·Canadian Journal of Physiology and Pharmacology·F LebrunN M Griffiths
Jan 30, 2003·American Journal of Physiology. Renal Physiology·Li E YangA A McDonough
Nov 1, 1990·The British Journal of Nutrition·A J TurnbullR P Thompson
May 12, 1998·The American Journal of Physiology·Y ZhangA A McDonough
Oct 22, 2002·American Journal of Physiology. Renal Physiology·Li E YangA A McDonough
Aug 10, 1999·Biopharmaceutics & Drug Disposition·H Mukai, A Morino
Jun 11, 1999·The American Journal of Physiology·C B ThompsonA A McDonough
Sep 9, 1998·The American Journal of Physiology·R W FreelN D Vaziri
Mar 7, 2003·Pediatric Research·Jirí PáchaJana Bryndová
Mar 27, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ming Z FanDouglas G Burrin
Jan 1, 1980·CRC Critical Reviews in Biochemistry·J E Lever
Mar 17, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elizabeth A MarcusOlga Vagin
Aug 5, 2000·American Journal of Physiology. Renal Physiology·C E MagyarA A McDonough
Jun 23, 1995·The Journal of Biological Chemistry·M M Sánchez del PinoD R Peterson
Dec 14, 2017·International Journal of Molecular Sciences·Marco SalerGiulia Gastaldi
Dec 1, 1977·The American Journal of Physiology·U Hopfer
Feb 1, 1992·The American Journal of Physiology·A K MircheffA A McDonough
Nov 1, 1978·The American Journal of Physiology·M W WallingE M Wright
Dec 9, 1998·The American Journal of Physiology·F LebrunN M Griffiths
Sep 1, 1995·The American Journal of Physiology·C E MagyarA A McDonough

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