The surprising structural and mechanistic dichotomy of membrane-associated phosphoglycosyl transferases.

Biochemical Society Transactions
Katherine H O'TooleBarbara Imperiali

Abstract

Phosphoglycosyl transferases (PGTs) play a pivotal role at the inception of complex glycoconjugate biosynthesis pathways across all domains of life. PGTs promote the first membrane-committed step in the en bloc biosynthetic strategy by catalyzing the transfer of a phospho-sugar from a nucleoside diphospho-sugar to a membrane-resident polyprenol phosphate. Studies on the PGTs have been hampered because they are integral membrane proteins, and often prove to be recalcitrant to expression, purification and analysis. However, in recent years exciting new information has been derived on the structures and the mechanisms of PGTs, revealing the existence of two unique superfamilies of PGT enzymes that enact catalysis at the membrane interface. Genome neighborhood analysis shows that these superfamilies, the polytopic PGT (polyPGT) and monotopic PGT (monoPGT), may initiate different pathways within the same organism. Moreover, the same fundamental two-substrate reaction is enacted through two different chemical mechanisms with distinct modes of catalysis. This review highlights the structural and mechanistic divergence between the PGT enzyme superfamilies and how this is reflected in differences in regulation in their varied glycoconju...Continue Reading

References

Jun 5, 1993·Journal of Molecular Biology·S D Pickett, M J Sternberg
Jan 8, 1999·Biochimica Et Biophysica Acta·M A Kukuruzinska, K Lennon-Hopkins
Apr 14, 1999·Molecular Microbiology·C Whitfield, I S Roberts
Jul 6, 2000·Chemistry and Physics of Lipids·T JanasT Janas
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Miguel A Valvano
Feb 1, 2005·Biochimica Et Biophysica Acta·Lingyi Lynn DengJ David Carroll
Mar 9, 2005·Molecular Microbiology·Dennis LintonBrendan W Wren
Apr 22, 2005·Glycobiology·Neil P Price, Frank A Momany
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kerney Jebrell GloverBarbara Imperiali
Apr 8, 2009·Biochimica Et Biophysica Acta·Meredith B JonesSharon S Krag
Aug 10, 2010·The Journal of Biological Chemistry·Pritam K SenguptaMaria A Kukuruzinska
Sep 25, 2010·Biochemical Society Transactions·Emily KayBrendan W Wren
Mar 23, 2011·Biochemical and Biophysical Research Communications·Liliana Surmacz, Ewa Swiezewska
Sep 6, 2011·Nucleic Acids Research·Mikhail A LomizeAndrei L Lomize
Nov 19, 2011·Archives of Biochemistry and Biophysics·Meredith D Hartley, Barbara Imperiali
Jan 26, 2012·Science Signaling·Christine L GeeTom Alber
Apr 5, 2012·Proceedings of the National Academy of Sciences of the United States of America·Helgo SchmidtJeroen R Mesters
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Feb 11, 2015·The Journal of Biological Chemistry·Chiara TonioloRobert Janulczyk
Oct 3, 2015·FEMS Microbiology Reviews·Sylvie ManuseChristophe Grangeasse
Mar 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dan DavidiRon Milo
May 27, 2016·The Journal of Biological Chemistry·Yao LiuMaarten R Egmond
Aug 16, 2016·Current Opinion in Structural Biology·Karen N Allen, Debra Dunaway-Mariano
Aug 26, 2016·Glycobiology·Ajit Varki

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