The survivability of Mycoplasma meleagridis in frozen-thawed turkey semen

Poultry Science
W T FerrierR Yamamoto


Semen from 15 turkeys was pooled and divided into two groups and diluted with glycerated freezing medium. One group contained naturally occurring levels of Mycoplasma meleagridis while the other was inoculated with a 24-hr culture of the organism. Both groups were frozen under identical conditions. Semen was evaluated for motility and life-dead analysis during various stages of cryopreservation. The number of viable mycoplasmas in both groups was determined prior to and at 1-, 2-, and 6-months frozen storage. The experiment was repeated twice. Semen motility and live-dead evaluation showed no apparent trends outside of the normal decline seen during various stages of cryopreservation. There was no significant decline in M. meleagridis levels in either treatment group in either trial when samples were tested at intervals up to 6 months. The naturally infected semen contained approximately 10(3) cfu/ml, while the inoculated semen had 10(5) cfu/ml. It was concluded that viable numbers of M. meleagridis do not substantially decline in turkey semen during cryopreservation and subsequent thawing. Consideration must be given to potential pathogens in turkey semen cryopreserved for long-term storage.

Related Concepts

Mycoplasma putrefaciens
Seminal Plasma
Sperm Preservation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.