PMID: 7545851Apr 1, 1995Paper

The survival and growth of ovine afferent lymph dendritic cells in culture depends on tumour necrosis factor-alpha and is enhanced by granulocyte-macrophage colony-stimulating factor but inhibited by interferon-gamma

Veterinary Immunology and Immunopathology
D M HaigD Sargan

Abstract

An in vitro culture system is described which allows an analysis of the signals responsible for the survival, growth and functional maturation of afferent lymph dendritic cells (ALDC), a subpopulation of migrating dermal dendritic cells involved in antigen carriage and presentation to T-cells. Purified ALDC survived and grew for up to 30 days in lymph node conditioned medium and survived 14 days in recombinant ovine (rov) TNF-alpha whereas none were detected after 24 h in rov GM-CSF, rov IFN-gamma or rh M-CSF. However, when rov GM-CSF was added to cultures along with rov TNF-alpha, increased numbers of ALDC compared with input numbers (growth) were recorded on Days 14 and 21. In contrast, when 50-200 units ml-1 of rov IFN-gamma were added to cultures of ALDC along with TNF-alpha or rov TNF-alpha plus rov GM-CSF, cell survival and growth was inhibited. Antibody blocking studies confirmed the cytokine specificity of these effects. ALDC cultured in rov TNF-alpha or rov TNF-alpha plus rov GM-CSF retained MHC Class-II and ov CD-1 antigen expression and accessory function for autologous ov CD-4 T-cell proliferation, although at reduced levels compared with freshly isolated cells. Neither fresh nor cultured ALDC expressed coagulation ...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·K InabaG Schuler
Jun 1, 1992·Veterinary Immunology and Immunopathology·G EntricanD M Haig
Jan 1, 1991·Annual Review of Immunology·R M Steinman
May 1, 1990·Veterinary Immunology and Immunopathology·P R WoodS L Jones
Nov 1, 1990·The Journal of Experimental Medicine·C P LarsenJ M Austyn
Mar 1, 1990·The Journal of Experimental Medicine·C R MackayL Dudler
Jan 1, 1990·Transplantation·J M Austyn, C P Larsen
Nov 1, 1988·European Journal of Immunology·C R MackayP Matzinger
Oct 1, 1989·The British Journal of Dermatology·R CerioJ T Headington
Oct 1, 1989·The Journal of Experimental Medicine·R BujdosoI McConnell
Sep 3, 1987·Nature·S E AdamsA J Kingsman
Jan 1, 1987·International Archives of Allergy and Applied Immunology·W R HeinM Miyasaka
Mar 1, 1985·The Journal of Experimental Medicine·G Schuler, R M Steinman
Nov 1, 1993·Veterinary Immunology and Immunopathology·C J HowardK R Parsons
Nov 1, 1993·Veterinary Immunology and Immunopathology·V K GuptaJ Hopkins

❮ Previous
Next ❯

Citations

Mar 1, 1996·Veterinary Immunology and Immunopathology·G EntricanD M Haig
Nov 1, 2001·Developmental and Comparative Immunology·Charles A WestSteven J Mentzer
Jun 20, 2002·Veterinary Immunology and Immunopathology·David M HaigStephen Fleming
Sep 26, 2002·Virus Research·David M Haig, Colin J McInnes
Jan 26, 1999·Cytokine & Growth Factor Reviews·A Rice, T Chard
May 8, 1999·Immunology·D M HaigH R Miller
Mar 21, 2002·Immunology and Cell Biology·G J ClydesdaleH K Muller
Oct 12, 2000·Journal of Virology·S RyanB Blacklaws
Mar 26, 2015·Viruses·Stephen B FlemingAndrew A Mercer
Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.