Abstract
Schistosomiasis is a chronic disease caused by trematode flatworms of the genus Schistosoma and its control is dependent on a single drug, praziquantel (PZQ), but concerns over PZQ resistance have renewed interest in evaluating the in vitro susceptibility of recent isolates of Schistosoma mansoni to PZQ in comparison with well-established strains in the laboratory. The in vitro activity of PZQ (6.5-0.003 µg/mL) was evaluated in terms of mortality, reduced motor activity and ultrastructural alterations against S. mansoni. After 3 h of incubation, PZQ, at 6.5 µg/mL, caused 100% mortality of all adult worms in the three types of recent isolates, while PZQ was inactive at concentrations of 0.08-0.003 µg/mL after 3 h of incubation. The results show that the SLM and Sotave isolates basically presented the same pattern of susceptibility, differing only in the concentration of 6.5 µg/mL, where deaths occurred from the range of 1.5 h in Sotave and just in the 3 h range of SLM. Additionally, this article presents ultrastructural evidence of rapid severe PZQ-induced surface membrane damage in S. mansoni after treatment with the drug, such as disintegration, sloughing, and erosion of the surface. According to these results, PZQ is very eff...Continue Reading
References
Dec 29, 2000·The Journal of Parasitology·S XiaoM Tanner
Aug 14, 2001·The Journal of Biological Chemistry·A B KohnR M Greenberg
Aug 22, 2001·International Journal for Parasitology·Y S LiangV R Southgate
Aug 23, 2005·Memórias do Instituto Oswaldo Cruz·Iran Mendonça da SilvaPaulo César Ribeiro
Jun 23, 2006·The Lancet Infectious Diseases·Peter SteinmannJürg Utzinger
Mar 18, 2008·Nature Medicine·Ahmed A SayedDavid L Williams
Nov 4, 2008·Current Opinion in Infectious Diseases·Michael J DoenhoffJürg Utzinger
Dec 17, 2008·Revista Do Instituto De Medicina Tropical De São Paulo·Iran Mendonça da SilvaHenrique Leonel Lenzi
Dec 23, 2008·Molecular and Biochemical Parasitology·Anthony D AragonCharles Cunningham
Jan 23, 2009·PLoS Neglected Tropical Diseases·Thorsten MeyerChristian Miculka
May 12, 2009·Acta Tropica·Livia Pica-MattocciaDonato Cioli
Jan 5, 2011·Immunological Reviews·Jeffrey M BethonyPeter J Hotez
May 4, 2011·Memórias do Instituto Oswaldo Cruz·Flávia F B CoutoAna Carolina A Mattos
May 4, 2011·Revista Da Sociedade Brasileira De Medicina Tropical·Caliandra Maria Bezerra Luna LimaMargareth de Fátima Formiga Melo Diniz
Oct 12, 2012·Parasitology Research·Wei WangYou-Sheng Liang
Aug 16, 2013·Planta medica·Thiago José Matos-RochaFábio André Brayner
Citations
Mar 23, 2017·Revista Da Sociedade Brasileira De Medicina Tropical·Thiago José Matos-RochaFábio André Brayner
Jul 12, 2019·Scientific Reports·Nattapon SimanonOnrapak Reamtong
Sep 23, 2020·Parasitology·Rayan Rubens da Silva AlvesGuilherme Silva Miranda
Dec 3, 2020·Parasitology Research·Franziska WinkelmannMartina Sombetzki
Oct 30, 2020·Infection and Drug Resistance·Amany Ibrahim AmmarAmany Ahmed Rady
Jan 29, 2020·Acta Tropica·Juliana Carla Serafim SilvaBruno Melo Carvalho