The sustained release of basic fibroblast growth factor accelerates angiogenesis and the engraftment of the inactivated dermis by high hydrostatic pressure

PloS One
Tien Minh LeKenji Kusumoto

Abstract

We developed a novel skin regeneration therapy combining nevus tissue inactivated by high hydrostatic pressure (HHP) in the reconstruction of the dermis with a cultured epidermal autograft (CEA). The issue with this treatment is the unstable survival of CEA on the inactivated dermis. In this study, we applied collagen/gelatin sponge (CGS), which can sustain the release of basic fibroblast growth factor (bFGF), to the inactivated skin in order to accelerate angiogenesis. Murine skin grafts from C57BL6J/Jcl mice (8 mm in diameter) were prepared, inactivated by HHP and cryopreserved. One month later, the grafts were transplanted subcutaneously onto the back of other mice and covered by CGS impregnated with saline or bFGF. Grafts were harvested after one, two and eight weeks, at which point the engraftment was evaluated through the histology and angiogenesis-related gene expressions were determined by real-time polymerase chain reaction. Histological sections showed that the dermal cellular density and newly formed capillaries in the bFGF group were significantly higher than in the control group. The relative expression of FGF-2, PDGF-A and VEGF-A genes in the bFGF group was significantly higher than in the control group at Week 1....Continue Reading

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Citations

Oct 23, 2019·Advances in Wound Care·Pinghui ZhouWenguo Cui
Oct 21, 2020·Scientific Reports·Tien Minh LeKenji Kusumoto
Nov 6, 2020·Growth Factors·Sanam DolatiSeyed Kazem Shakouri
Jun 12, 2021·Regenerative Medicine·Fei SunHongcan Shi
Oct 3, 2021·Tissue Engineering. Part a·Yuki HasegawaHiroyuki Sakurai

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