The swine steroid 21-hydroxylase gene (CYP21): cloning and mapping within the swine leucocyte antigen complex

Animal Genetics
C GeffrotinM Vaiman

Abstract

A swine genomic cosmid library constructed from a genotypically SLA homozygous Large White individual was screened with a murine genomic 21-hydroxylase probe. A clone which contained a pig 21-hydroxylase gene was isolated and after subcloning, the 5' region of the gene was sequenced. The deduced amino acid sequence corresponded almost exactly to the NH2 terminal portion of the steroid 21-hydroxylase from porcine adrenal microsomes. Comparison of the first 99 amino acid residues of both sequences revealed three substitutions comprising two leucine residues in positions 10 and 13, and one arginine residue in position 55 for our sequence, instead of threonine in position 10 and lysine in position 13 and 55 for the isolated enzyme. A swine homologous probe was derived from the isolated 21-hydroxylase gene and used for gene assignment by RFLP studies in two swine leucocyte antigen (SLA) informative families. The results demonstrate that the swine 21-hydroxylase gene is located within or close to the swine MHC. Taken together, the present results suggest the existence of a single 21-hydroxylase gene per haploid genome.

References

Jun 5, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P Iványi
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1988·Endocrinology·H GotohK Moriwaki
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y HigashiY Fujii-Kuriyama
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B C ChungW L Miller
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P C WhiteB Dupont
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D D ChaplinK L Parker
Apr 1, 1987·Archives of Biochemistry and Biophysics·M HaniuJ E Shively
Jan 1, 1986·Gene·S H Cross, P F Little
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M C CarrollR R Porter
Jun 1, 1982·American Journal of Reproductive Immunology : AJRI·M SimonsenP Sørensen

❮ Previous
Next ❯

Citations

Jan 1, 1991·Immunogenetics·P ChardonM Vaiman
Sep 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L WuC M Warner
Oct 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M NunesP Chardon
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M NunesM Vaiman
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Y XuC M Warner
Oct 1, 1994·Veterinary Immunology and Immunopathology·J K Lunney
Dec 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·W L MillerY Morel
Nov 6, 2010·Endocrine Reviews·Walter L Miller, Richard J Auchus
May 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L J PeelmanR D Campbell
Jan 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M YerleC Moran
Sep 13, 2006·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·B BuskeG Brockmann
Sep 12, 2018·Critical Reviews in Toxicology·D LisonG Van Maele-Fabry
Jun 1, 1993·Animal Genetics·L AnderssonL B Schook
Nov 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M NunesC Renard
Jun 1, 1996·Animal Genetics·M YerleC Renard
Mar 20, 2003·Journal of Animal Science·R QuintanillaM Bonneau
Jan 29, 2021·Frontiers in Immunology·Walter L Miller
May 1, 1992·Molecular and Cellular Biology·S E GitelmanW L Miller
May 8, 2018·Hormone Research in Pædiatrics·Walter L Miller, Deborah P Merke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
D D ChaplinK L Parker
Proceedings of the National Academy of Sciences of the United States of America
Y HigashiY Fujii-Kuriyama
Proceedings of the National Academy of Sciences of the United States of America
P C WhiteB Dupont
© 2021 Meta ULC. All rights reserved