The switch in the helical handedness of the histone (H3-H4)2 tetramer within a nucleoprotein particle requires a reorientation of the H3-H3 interface.

The Journal of Biological Chemistry
A Hamiche, H Richard-Foy

Abstract

It has recently been proposed that the histone (H3-H4)2 tetramer undergoes structural changes, which allow the particle to accommodate both negatively and positively constrained DNA. To investigate this process, we modified histone H3 at the H3-H3 interface, within the histone (H2A-H2B-H3-H4)2 octamer or the histone (H3-H4)2 tetramer, by forming adducts on the single cysteine of duck histone H3. We used three sulfhydryl reagents, iodoacetamide, N-ethylmaleimide, and 5,5'-dithiobis(2-nitrobenzoic acid). Torsionally constrained DNA was assembled on the modified histones. The H3 adducts, which have no effect on the structure of the nucleosome, dramatically affected the structural transitions that the (H3-H4)2 tetrameric nucleoprotein particle can undergo. Iodoacetamide and N-ethylmaleimide treatment prevented the assembly of positively constrained DNA on the tetrameric particle, whereas 5, 5'-dithiobis(2-nitrobenzoic acid) treatment strongly favored it. Determination of DNA topoisomer equilibrium after relaxation of the tetrameric nucleoprotein particles with topoisomerase I demonstrated that the structural transition occurs without histone dissociation. Incorporation of H2A-H2B dimers into the tetrameric particle containing modif...Continue Reading

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E Depew, J C Wang
Jul 1, 1977·Cell·M Bina-Stein, R T Simpson
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·W R Bauer
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·D E PulleyblankH P Vosberg
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M S Lee, W T Garrard
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G ArentsE N Moudrianakis
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F Dong, K E van Holde
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L F Liu, J C Wang
Mar 20, 1988·Journal of Molecular Biology·Y ZivanovicA Prunell
Apr 10, 1987·Nucleic Acids Research·I GouletA Prunell
Jun 18, 1985·Biochemistry·L Louters, R Chalkley
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Feb 15, 1984·Journal of Molecular Biology·D S Horowitz, J C Wang
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J C WangK Becherer
Oct 11, 1984·Nature·T J RichmondA Klug
Apr 25, 1982·Journal of Molecular Biology·J R Daban, C R Cantor
Oct 1, 1981·European Journal of Biochemistry·D L BatesJ O Thomas
Jun 7, 1994·Biochemistry·M GersteinC Chothia
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·G Arents, E N Moudrianakis
Jun 8, 1993·Biochemistry·V Jackson
Mar 22, 1996·Journal of Molecular Biology·A HamicheA Prunell
Jan 1, 1996·Advances in Protein Chemistry·R A GraylingJ N Reeve
Dec 19, 1996·Nature·A J Bannister, T Kouzarides
May 1, 1997·Nature·A P Wolffe
Jun 27, 1997·Cell·J N ReeveC J Daniels
Jan 1, 1972·Methods in Enzymology·J F Riordan, B L Vallee
Jan 1, 1972·Methods in Enzymology·A F Habeeb

❮ Previous
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Citations

May 4, 2012·Chromosoma·Steven Henikoff, Takehito Furuyama
May 24, 2005·Journal of Molecular Biology·Kirit R Pindolia, Leonard C Lutter
Nov 26, 2002·Trends in Genetics : TIG·Malcolm F White, Stephen D Bell
Jan 29, 2000·Molecular Microbiology·D MusgraveA Slesarev
Dec 24, 1998·Nucleic Acids Research·K A BaileyJ N Reeve
Sep 13, 2000·Molecular and Cellular Biology·V Morales, H Richard-Foy
Nov 30, 2005·Molecular and Cellular Biology·Natalia P Ulyanova, Gavin R Schnitzler
Mar 31, 2007·Biochimica Et Biophysica Acta·Joke J F A van VugtColin Logie
Nov 12, 2009·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Makoto SakamotoMasami Horikoshi
Dec 15, 2010·Molecular Microbiology·Betty M BookerN Patrick Higgins
Jun 29, 2004·Trends in Genetics : TIG·Steven HenikoffKami Ahmad
Feb 17, 2009·Structure·Jordanka ZlatanovaKen van Holde
Aug 13, 2004·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Andrei Sivolob, Ariel Prunell
May 29, 1999·The Journal of Biological Chemistry·U G SathyanarayanaW T Garrard
Dec 26, 2001·The Journal of Biological Chemistry·Kathryn A BaileyJohn N Reeve
Jun 12, 2002·The Journal of Biological Chemistry·Frederic MarcJohn N Reeve
Jul 31, 2003·The Journal of Biological Chemistry·Caroline DüringerCatharina Svanborg
Apr 2, 2018·The Journal of Chemical Physics·Orkide OrduNynke H Dekker
Sep 16, 2019·Biophysical Journal·Orkide OrduNynke H Dekker
Mar 8, 2000·Journal of Molecular Biology·D J SoaresJ N Reeve

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