The symbiotically essential cbb(3)-type oxidase of Bradyrhizobium japonicum is a proton pump

FEBS Letters
E ArslanH Hennecke

Abstract

Purified cbb(3)-type oxidase of Bradyrhizobium japonicum was reconstituted into phospholipid vesicles. Tight vesicles were obtained as shown by the disturbance of deltapH with CCCP and the membrane potential with valinomycin, which led to a six-fold increase in cytochrome c oxidase activity. The vesicles were thus suitable for proton translocation experiments. In the presence of valinomycin, a pulse with reduced cytochrome c caused an acidification with a subsequent alkalinization, whereas the same pulse caused only an alkalinization in the presence of valinomycin plus CCCP. We conclude that the cbb(3)-type oxidase of B. japonicum is a proton pump.

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Citations

Dec 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yafei HuangPia Adelroth
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Huazhi HanRobert B Gennis
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Hsin-Yang ChangRobert B Gennis
Oct 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hyun Ju LeePia Adelroth
Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Virve RauhamäkiM Wikström
Mar 26, 2010·The Journal of Biological Chemistry·Doris BühlerH Hennecke
Jul 21, 2004·Journal of Molecular Microbiology and Biotechnology·Ravi P TiwariJohn G Howieson
Nov 8, 2011·Biochimica Et Biophysica Acta·Hyun Ju LeePia Adelroth
Oct 18, 2011·Biochimica Et Biophysica Acta·Filipa L SousaM M Pereira
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Dec 7, 2007·The Journal of Biological Chemistry·Ulrika FlockPia Adelroth
Jun 19, 2002·The Journal of Biological Chemistry·Robert S PitcherNicholas J Watmough
Apr 6, 2005·Biochemistry. Biokhimii︠a︡·M S MuntyanV S Ustiyan

Related Concepts

Cytochrome bb3
Cytochrome FixP
Cytochrome FixO
FixQ protein, Bradyrhizobium japonicum
FixN protein, bacteria
Bacterial Proteins
Cytochrome c Group
Cytochrome c Oxidase Subunit VIa
Hydrogen-Ion Concentration
Resting Potentials

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