The synergistic effect of ethanol and shock insults on Caco-2 cytokine production and apoptosis

Shock
Parth B AminD M Liberati

Abstract

Gut epithelial cells are important in orchestrating immunoinflammatory responses in the gut and may impact systemic immunocompetent cells after shock and trauma. Ethanol (EtOH) intoxication is an important etiological factor in trauma and may increase the likelihood of posttraumatic septic complications. Both EtOH and gut I/R impair intestinal barrier function. However, their combined effects on intestinal epithelial cell function and barrier integrity are unknown. Confluent CaCO2 cell monolayers were grown in a two-chamber culture system and exposed to 0.1% EtOH and/or Escherichia coli C-25 under normoxic (21% O2) or hypoxia (5% O2) followed by reoxygenation (H/R). Apical and basal compartment supernatants were collected, and TNF and IL-6 were quantitated by enzyme-linked immunosorbent assay (picograms per milliliter). CaCO2 cell integrity was indexed by apoptosis and monolayer permeability. TNF-alpha production by CaCO2 cells are greatest when incubated with EtOH and then exposed to H/R group. The apical levels of TNF production are consistently higher than basal levels, although the trend toward increased cytokine production is similar in both compartments. IL-6 production by the CaCO2 cell is also greatest when CaCO2 cells ...Continue Reading

References

Mar 1, 1983·Infection and Immunity·C F DenekeS L Gorbach
Feb 7, 1998·Pharmaceutical Research·P Artursson, R T Borchardt
Oct 31, 2000·Microscopy Research and Technique·T A MillerE R Kokoska
Aug 25, 2001·Journal of Endotoxin Research·M AckermannF U Schade
Sep 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·X ZhaoD L Carden
Dec 13, 2002·Antisense & Nucleic Acid Drug Development·Jeffrey D KirschArthur M Krieg
Sep 16, 2003·Critical Care Medicine·Fabienne TamionGuy Bonmarchand
Dec 16, 2005·The Journal of Trauma·Larry M Gentilello
Dec 16, 2005·The Journal of Trauma·Ernest E Moore
May 18, 2007·American Journal of Respiratory and Critical Care Medicine·Mary Y YehLou Ann S Brown

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis