The synergistic effect of micro-topography and biochemical culture environment to promote angiogenesis and osteogenic differentiation of human mesenchymal stem cells

Acta Biomaterialia
Shang SongShuvo Roy

Abstract

Critical failures associated with current engineered bone grafts involve insufficient induction of osteogenesis of the implanted cells and lack of vascular integration between graft scaffold and host tissue. This study investigated the combined effects of surface microtextures and biochemical supplements to achieve osteogenic differentiation of human mesenchymal stem cells (hMSCs) and revascularization of the implants in vivo. Cells were cultured on 10μm micropost-textured polydimethylsiloxane (PDMS) substrates in either proliferative basal medium (BM) or osteogenic medium (OM). In vitro data revealed that cells on microtextured substrates in OM had dense coverage of extracellular matrix, whereas cells in BM displayed more cell spreading and branching. Cells on microtextured substrates in OM demonstrated a higher gene expression of osteoblast-specific markers, namely collagen I, alkaline phosphatase, bone sialoprotein, and osteocalcin, accompanied by substantial amount of bone matrix formation and mineralization. To further investigate the osteogenic capacity, hMSCs on microtextured substrates under different biochemical stimuli were implanted into subcutaneous pockets on the dorsal aspect of immunocompromised mice to study cap...Continue Reading

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Citations

Feb 13, 2016·Journal of Biomedical Materials Research. Part a·Tongqiang ZouYubo Fan
Sep 23, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ming DingSøren Overgaard
May 17, 2018·Biomaterials Science·Mahboubeh JafarkhaniMasoud Mozafari
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Jan 1, 2020·Progress in Biomedical Engineering·Guang YangJohn P Fisher
Dec 4, 2021·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ping Xia, Yongxiang Luo

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