The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus

Evidence-based Complementary and Alternative Medicine : ECAM
Prairadda CheypratubG Eumkeb

Abstract

Cyperus rotundus L. has been used for pharmaceutical applications including antibacterial infections. Nevertheless, there is still no data regarding the mode of actions. This study aimed to determine the antibacterial activity and mode of actions of Cyperus rotundus extract (CRE) against ampicillin-resistant Staphylococcus aureus (ARSA) which poses a serious problem for hospitalized patients. The majority of chemical compounds of CRE were flavonoids and alkaloids. The minimum inhibitory concentrations (MICs) for ampicillin and CRE against all ARSA strains were 64 μg/ml and 0.5 mg/ml, respectively. Checkerboard assay revealed synergistic activity in the combination of ampicillin and CRE at the lowest fractional inhibitory concentration index (FICI) at 0.27. The killing curve assay had confirmed the synergistic and bactericidal activity of the combination against ARSA. Electron microscopic results showed that these ARSA cells treated with this combination caused peptidoglycan and cytoplasmic membrane (CM) damage and average cell areas significantly smaller than control. Also, this combination caused an increase in CM permeability of ARSA. CRE revealed the inhibitory activity against β-lactamase. It is normally known that some dru...Continue Reading

References

Jun 8, 2000·Journal of Natural Products·S J JeongR Higuchi
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·UNKNOWN European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and In
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·UNKNOWN European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and In
Jan 15, 2003·Journal of Mass Spectrometry : JMS·Ferran Sánchez-RabanedaRosa M Lamuela-Raventós
Jun 14, 2003·The Journal of Antimicrobial Chemotherapy·F C Odds
Nov 9, 2004·The Journal of Antimicrobial Chemotherapy·Alexander John O'NeillIan Chopra
May 11, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S P Voravuthikunchai, L Kitpipit
Jan 1, 1997·International Journal of Antimicrobial Agents·L S TzouvelekisN J Legakis
Nov 3, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Griangsak EumkebSupatcharee Siriwong
Aug 17, 2011·International Journal of Molecular Sciences·Olayinka AiyegoroAnthony Okoh
Nov 20, 2012·Journal of Photochemistry and Photobiology. B, Biology·G EumkebK Thumanu
Dec 12, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Griangsak Eumkeb, Somnuk Chukrathok
Jan 22, 2013·Trends in Biotechnology·Roberta J Worthington, Christian Melander
Mar 1, 2013·Microbial Biotechnology·Patricia BernalMaría A Llamas
Dec 18, 2013·Phytotherapy Research : PTR·Shuang-Ying WangMu Qing
Apr 17, 2015·Journal of Ayurveda and Integrative Medicine·M NagarajanPadma Venkatasubramanian
Nov 18, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Supatcharee SiriwongGriangsak Eumkeb

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