PMID: 5914690Feb 1, 1966

The synthesis of DNA, RNA, and nuclear protein in normal and tumor strain cells. I. Fresh embryo human cells

The Journal of Cell Biology
J SEED

Abstract

Interferometric and photometric measurements have been made on replicating embryo human cell cultures. From a study of the relations between successive physical measurements on individual cells, it was found that the net syntheses of DNA, nuclear RNA, nuclear protein, and cytoplasmic RNA are closely associated during interphase. Additional measurements of DNA and cytoplasmic RNA on freshly prepared replicating monkey kidney cells gave similar results. In auxiliary experiments with embryo human cells, an inhibition of the onset of DNA synthesis (produced by a dose of X-rays) was found to block the majority of the accumulation of nuclear protein and RNA and about half the accumulation of cytoplasmic RNA. These results are consistent with others previously reported in dividing cell cultures freshly prepared from normal tissues.

References

Oct 16, 1952·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P M B WALKER, H B YATES
Oct 1, 1953·J R Microsc Soc·R J KING, E M ROE
Mar 14, 1956·Annals of the New York Academy of Sciences·B M RICHARDSE M DEELEY
Oct 1, 1957·Experimental Cell Research·R BARER, S JOSEPH
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD
Aug 1, 1961·Proceedings of the National Academy of Sciences of the United States of America·M YAMADA, T T PUCK
Apr 1, 1960·Experimental Cell Research·L E FEINENDEGENR B Painter
Apr 1, 1960·Experimental Cell Research·M ZALOKAR
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·L GOLDSTEIN, J MICOU
Oct 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·J BONNERN MAHESHWARI
Dec 16, 1964·Biochimica Et Biophysica Acta·H J LIN, E CHARGAFF
Mar 29, 1952·Nature·H G DAVIES, M H F WILKINS

Citations

Apr 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U K Ehmann, K T Wheeler
Jan 1, 1977·Progress in Histochemistry and Cytochemistry·G Thiessen, H Thiessen
Dec 1, 1968·The Journal of Obstetrics and Gynaecology of the British Commonwealth·J S Mitchell

Related Concepts

Cell Division Phases
Cell Nucleus
DNA, Double-Stranded
Embryonic Structures, Mammalian
HeLa Cells
Photometry
Effects of Radiation
RNA
Protein Biosynthesis

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