PMID: 5914693Feb 1, 1966

The synthesis of DNA, RNA, and nuclear protein in normal and tumor strain cells. IV. HeLa tumor strain cells

The Journal of Cell Biology
J SEED

Abstract

Interferometric and photometric measurements have been made on HeLa cells, a strain of cells originally derived from a human carcinoma. From a study of the relations between successive physical measurements on individual cells, it was confirmed that, whereas the net syntheses of nuclear RNA and nuclear protein are closely associated during interphase, they are dissociated from DNA replication to a significant extent. These results on nuclear metabolism agree with others previously reported in cell strains derived from tumors; they contrast with results from freshly prepared normal cells, where the net syntheses of DNA, nuclear RNA, and protein are closely associated during interphase. Cytoplasmic measurements on HeLa cells showed that much of the net synthesis of cytoplasmic RNA is associated with DNA replication as in normal cells, and they failed to detect transfer from the nucleus of a stable RNA component synthesized independently from DNA replication. In auxiliary experiments, an inhibition of the onset of DNA synthesis was produced by a dose of X-rays; under these conditions it was shown that the major part of the accumulation of nuclear protein was independent of DNA replication and that the accumulation of nuclear RNA w...Continue Reading

References

Feb 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·B D HALL, S SPIEGELMAN
Apr 1, 1960·Experimental Cell Research·L E FEINENDEGENR B PAINTER
Apr 1, 1960·Experimental Cell Research·M ZALOKAR
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·L GOLDSTEIN, J MICOU
Dec 1, 1961·Experimental Cell Research·L E FEINENDEGENW L HUGHES
Oct 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·M HAYASHI, S SPIEGELMAN
May 15, 1962·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H HARRIS, J W WATTS
Dec 16, 1964·Biochimica Et Biophysica Acta·H J LIN, E CHARGAFF

Citations

Dec 18, 2001·Environmental and Molecular Mutagenesis·J E Cleaver
Jan 1, 1970·Zeitschrift für Zellforschung und mikroskopische Anatomie·P Comes, W Franke
Apr 1, 1979·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·U K Ehmann, K T Wheeler
Apr 1, 1971·European Journal of Biochemistry·P R Harrison

Related Concepts

Cell Nucleus
Protoplasm
DNA, Neoplasm
HeLa Cells
Neoplasm Proteins
Photometry
Effects of Radiation
RNA, Neoplasm

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