The synthetic antimicrobial peptide pexiganan and its nanoparticles (PNPs) exhibit the anti-helicobacter pylori activity in vitro and in vivo

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Xiao-Lin ZhangJian-Fei Wang

Abstract

The aim of this study was to probe the potential anti-H. pylori activity of the synthetic antimicrobial peptide pexiganan, which is an analog of the peptide magainin, and its nanoparticles (PNPs) that were prepared in our laboratory. To compare their antibacterial effects in vitro and in vivo, studies of H. pylori growth inhibition, kinetics and resistance assays were undertaken. The gastric mucoadhesive efficiency and H. pylori clearance efficiency of pexiganan and PNPs were evaluated in rats and mice infected with H. pylori. The eradication of H. pylori was determined using urease tests and a microbial culture method. We observed that PNPs adhered to gastric mucosa more effectively owing to a prolonged stay in the stomach, which resulted in a more effective H. pylori clearance. In addition, PNPs had greater anti-H. pylori effect than pexiganan in infected mice. The amount of pexiganan required to eradicate H. pylori was significantly less using PNPs than the corresponding pexiganan suspension. The results confirmed that PNPs improved peptide stability in the stomach and more effectively eradicated H. pylori from mice stomachs than pexiganan.

References

Jul 1, 1993·Antimicrobial Agents and Chemotherapy·M P CooremanK J Hengels
Oct 3, 1998·Antimicrobial Agents and Chemotherapy·N NagaharaY Ogawa
Jan 8, 1999·Drugs·H M Lamb, L R Wiseman
Apr 2, 1999·Antimicrobial Agents and Chemotherapy·Y GeM Zasloff
Jul 27, 1999·Archives of Internal Medicine·M B FennertyM Helfand
Jun 7, 2000·The Biochemical Journal·M P DeaconS E Harding
May 10, 2001·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·M CuñaD Torres
Feb 19, 2002·Antimicrobial Agents and Chemotherapy·Shiri Navon-VeneziaAmram Mor
Dec 13, 2005·Current Opinion in Immunology·Kelly L Brown, Robert E W Hancock
May 11, 2006·Journal of the American Chemical Society·Sergey DvinskikhAyyalusamy Ramamoorthy
Jun 17, 2006·Nature Reviews. Microbiology·Andreas Peschel, Hans-Georg Sahl
Jul 25, 2006·Expert Opinion on Investigational Drugs·Jamie S Mader, David W Hoskin
Oct 17, 2006·Clinical Microbiology Reviews·Holly M Scott Algood, Timothy L Cover
Feb 21, 2007·Cellular and Molecular Life Sciences : CMLS·N Mookherjee, R E W Hancock
Apr 7, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rania A H IshakSamia A K Nour
May 29, 2007·Nature Biotechnology·Inna S RadzishevskyAmram Mor
Apr 4, 2008·Molecular Medicine·Lars SteinstraesserTobias Hirsch
Oct 7, 2008·Acta Cytologica·Amir Ahmad MostaghniPerikala Kumar
Jul 16, 2009·Current Pharmaceutical Design·Gill DiamondKevin O Kisich
Nov 10, 2010·PLoS Pathogens·Brian M PetersMary Ann Jabra-Rizk

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Citations

Jan 17, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hinojal ZazoJosé M Lanao
Nov 28, 2015·International Journal of Molecular Sciences·Xiaolin ZhangJianfei Wang
Mar 9, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Raffaela PeroOlga Scudiero
Nov 16, 2018·Helicobacter·Alireza NeshaniKiarash Ghazvini
Feb 8, 2020·Nanomedicine·Qianyu ZhangXuefeng Xia
Nov 2, 2017·Expert Review of Gastroenterology & Hepatology·Darren Michael MossAndrew Owen
Jul 11, 2018·Frontiers in Chemistry·Patrícia M CarvalhoMarco M Domingues
Apr 19, 2017·FEMS Microbiology Reviews·Michal Natan, Ehud Banin
Dec 5, 2019·Scientific Reports·Paula ParreiraM Cristina L Martins
Nov 20, 2020·Critical Reviews in Food Science and Nutrition·Xiaohong SunChibuike C Udenigwe
Nov 26, 2018·International Journal of Antimicrobial Agents·Guillaume André DurandGrégory Dubourg
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xinyu SongZhiyu He

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Methods Mentioned

BETA
NMR
Protein Assay

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