The synthetic cannabinoid WIN-55,212 induced-apoptosis in cytotrophoblasts cells by a mechanism dependent on CB1 receptor.

Toxicology
M AlmadaG Correia-da-Silva

Abstract

The endocannabinoid system has evolved as a key regulator in several pathological and physiological processes, including placentation, decidualization and implantation. In addition, it is known that Cannabis and cannabinoids negatively affect female reproduction. Although, the biological action of synthetic cannabinoids, such as WIN-55,212, in human fertility and pregnancy outcome remain to be unveiled. A tight balance between proliferation, differentiation and apoptosis of trophoblast cells is required for placental development and pregnancy outcome. Therefore, in this work, the effects of the synthetic cannabinoid WIN-55,212 in placental cytotrophoblast cells were explored. For that, it was used a human choriocarcinoma cell line, BeWo cells, and primary cultures of human cytotrophoblasts isolated from term placentas. Results demonstrate that this synthetic cannabinoid induces cell cycle arrest. We also observed that cell viability loss was associated with a disruption of mitochondrial membrane potential and activation of caspases -9 and -3/-7 independently of reactive oxygen species (ROS) production or recruitment of the endoplasmic reticulum stress marker CHOP. Moreover, these effects were prevented by pre-incubation with a ...Continue Reading

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Citations

Jun 9, 2020·Human & Experimental Toxicology·E M GulerA Kocyigit
Jul 11, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·João MaiaGeorgina Correia-da-Silva
Dec 8, 2020·Journal of Developmental Origins of Health and Disease·Oyekoya T AyonrindeOyekunle K Ayonrinde
Feb 6, 2020·Gynécologie, Obstétrique, Fertilité & Sénologie·P CastelB Courbiere
Aug 28, 2021·International Journal of Molecular Sciences·Annia A Martínez-PeñaSandeep Raha

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