The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn.

BMC Microbiology
Niall FoyKevin M Devine

Abstract

Lysyl-tRNA synthetase (LysRS) is unique within the aminoacyl-tRNA synthetase family in that both class I (LysRS1) and class II (LysRS2) enzymes exist. LysRS1 enzymes are found in Archaebacteria and some eubacteria while all other organisms have LysRS2 enzymes. All sequenced strains of Bacillus cereus (except AH820) and Bacillus thuringiensis however encode both a class I and a class II LysRS. The lysK gene (encoding LysRS1) of B. cereus strain 14579 has an associated T box element, the first reported instance of potential T box control of LysRS expression. A global study of 891 completely sequenced bacterial genomes identified T box elements associated with control of LysRS expression in only four bacterial species: B. cereus, B. thuringiensis, Symbiobacterium thermophilum and Clostridium beijerinckii. Here we investigate the T box element found in the regulatory region of the lysK gene in B. cereus strain 14579. We show that this T box element is functional, responding in a canonical manner to an increased level of uncharged tRNALys but, unusually, also responding to an increased level of uncharged tRNAAsn. We also show that B. subtilis strains with T box regulated expression of the endogenous lysS or the heterologous lysK gen...Continue Reading

References

Feb 1, 1989·Genes & Development·C Karmazyn-CampelliP Stragier
Aug 1, 1994·Molecular Microbiology·T M Henkin
Oct 1, 1993·European Journal of Pediatrics·U Blecker, Y Vandenplas
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·M IbbaD Söll
Jul 23, 1998·Journal of Applied Microbiology·L BaillieR Manchee
Dec 10, 1998·Microbiology·Valerie VagnerS Dusko Ehrlich
May 21, 1999·The Journal of Biological Chemistry·A BolhuisJ M van Dijl
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C R WoeseD Söll
May 29, 2001·EMBO Reports·M Ibba, D Söll
Mar 15, 1961·Proceedings of the National Academy of Sciences of the United States of America·C ANAGNOSTOPOULOS, I P CRAWFORD
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian C JesterKevin M Devine
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Patrick O'Donoghue, Zaida Luthey-Schulten
Aug 3, 2005·EMBO Reports·Sandro F AtaideMichael Ibba
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Mar 25, 2008·RNA·Alexey G VitreschakMikhail S Gelfand
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Ana Gutiérrez-PreciadoEnrique Merino
Nov 26, 2009·FEBS Letters·Nicholas J GreenTina M Henkin

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Methods Mentioned

BETA
aminoacylate
PCR

Software Mentioned

BLAST

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