The T-lymphocyte proliferative response to synthetic peptide antigens of defined secondary structure.

Scandinavian Journal of Immunology
S MouritsenO Werdelin

Abstract

Immunodominant sites in proteins recognized by T lymphocytes are segments consisting of at least 7-8 amino acids. It has previously been proposed that these sites in proteins are alpha-helical and amphipatic structures. We synthesized and investigated the immunogenicity of three synthetic peptides (MP7, MP8, and MP9), each consisting of the same 15 amino acids, but differing with respect to sequence. Based on information analysis and circular dichroism measurements, MP7 was shown to have an alpha-helical secondary structure and, based on previously assigned hydrophilicity indices, was also strongly longitudinally amphipatic. MP8 also was conformed as an alpha-helix, but was amphipatic in the sense that the N-terminal half of the molecule was hydrophilic and the C-terminal half hydrophobic. MP9 had neither an amphipatic nor alpha-helical structure. All three peptides were immunogenic in some strains of mice but none was immunogenic in all strains. This supports other studies concluding that amphipaticity per se is neither a necessary nor sufficient requirement for immunogenicity of a peptide. On the other hand, the present experimental data suggest that longitudinally amphipatic alpha-helical peptides may function better as T-ce...Continue Reading

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DeLisi, J A Berzofsky
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A SetteH M Grey
Aug 1, 1983·The Journal of Experimental Medicine·R ShimonkevitzH Grey
Dec 7, 1984·Journal of Chromatography·B A BidlingmeyerT L Tarvin
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
Next ❯

Citations

Oct 25, 2003·Biochemical and Biophysical Research Communications·Sarada SubramanianP Radhakantha Adiga
Aug 15, 1994·European Journal of Biochemistry·H E ChristensenJ Ulstrup
Jan 1, 1994·International Journal of Peptide and Protein Research·B D Larsen, A Holm
May 1, 1994·Infection and Immunity·S BirkelundG Christiansen
Jan 1, 1993·Infection and Immunity·J W PetersenI Heron
Jun 1, 1993·Infection and Immunity·P H IbsenI Heron
Oct 1, 1992·Infection and Immunity·J W PetersenI Heron

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.