PMID: 9634327Jun 20, 1998Paper

The tampon test for trichomoniasis: a comparison between conventional methods and a polymerase chain reaction for Trichomonas vaginalis in women

Sexually Transmitted Infections
B A PatersonF J Bowden

Abstract

Trichomonas vaginalis is the most common STD worldwide and the infection has been linked with an increased risk of HIV transmission. We present a detailed comparison between conventional collection and testing methods and the polymerase chain reaction (PCR) tampon test for T vaginalis. Women were tested for the presence of T vaginalis by PCR analysis of a tampon specimen and by conventional methods which included one or more of the following: culture and microscopy from a high vaginal swab (HVS) or endocervical swab (ECS), and microscopy of a Papanicolaou (Pap) smear. T vaginalis was detected in 51/590 (8.6%) conventional tests and 93/590 (15.8%) tampon specimens. Retesting of all tampon PCR positive specimens confirmed 89/93 (95.7%) tests. Using the tampon PCR as the reference, the sensitivities of the different conventional sampling and testing methods for the detection of T vaginalis were 8.3% (5/60) for ECS microscopy or culture, 31% (13/42) for HVS microscopy or culture, 52.8% (19/36) for HVS directly inoculated into Trichomonas medium and 59.4% (38/64) for Pap smear. No conventional test in the remote setting has comparable sensitivity to PCR. The Pap smear is the next most sensitive, but requires a speculum examination. ...Continue Reading

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Citations

Oct 19, 2004·Clinical Microbiology Reviews·Jane R Schwebke, Donald Burgess
Oct 2, 2002·Journal of Clinical Microbiology·Jane R Schwebke, Lisa F Lawing
Oct 12, 2000·Sexually Transmitted Infections·F J Bowden, G P Garnett
Apr 2, 2004·Annals of Family Medicine·Diane M HarperBernard F Cole
Mar 20, 2010·Journal of the European Academy of Dermatology and Venereology : JEADV·M DomeikaUNKNOWN Eastern European Network for Sexual and Reproductive Health
Sep 25, 2014·PloS One·Bertha Isabel Carvajal-GamezMaría Elizbeth Alvarez-Sánchez
Aug 24, 1999·Sexually Transmitted Diseases·M M HobbsI Hoffman
Apr 20, 2002·Current Opinion in Infectious Diseases·Michael W. Lehker, John F. Alderete
Apr 3, 2003·Sexually Transmitted Diseases·Elizabeth A SullivanJohn Kaldor
Mar 10, 2001·Diagnostic Cytopathology·I T Burja, M S Shurbaji
Mar 5, 2011·Sexual Health·Ruby N N UddinBasil Donovan
Aug 13, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Karen A WendelAnne M Rompalo
Oct 4, 2000·Journal of Clinical Microbiology·L F LawingJ R Schwebke

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