PMID: 7536799May 1, 1995Paper

The target cell nucleus is not required for cell-mediated granzyme- or Fas-based cytotoxicity

The Journal of Experimental Medicine
H NakajimaP A Henkart

Abstract

The requirement for target cell nuclei in the two apoptotic death pathways used by cytotoxic lymphocytes was tested using model effector systems in which the granzyme and Fas pathways of target damage are isolated. Mast cell tumors expressing granzymes A and B in addition to cytolysin/perforin lysed tumor target cells about 10-fold more efficiently than comparable effector cells without granzymes. Enucleated cytoplast targets derived from these cells were also lysed with a similar 10-fold effect of granzymes. In contrast to cytoplasts, effector granzyme expression did not influence lysis of red cell targets. The Fas pathway was assessed using the selected cytotoxic T lymphocyte hybridoma subline d11S, which lysed target cells expressing Fas but not those lacking Fas. Similarly, cytoplasts derived from Fas+ but not Fas- cells were also readily lysed by these effector cells. Thus, neither the nucleus itself nor the characteristic apoptotic nuclear damage associated with the two major cell death pathways used by cytotoxic lymphocytes are required for cell death per se.

References

Apr 7, 1975·Biochemical and Biophysical Research Communications·M H Wigler, I B Weinstein
Oct 8, 1992·Nature·R P BissonnetteD R Green
Sep 1, 1991·Molecular Immunology·D M OjciusJ D Young
Jan 1, 1989·Annual Review of Biochemistry·L F Liu
Jul 1, 1984·The Journal of Experimental Medicine·P A HenkartM P Henkart
Oct 1, 1994·The Journal of Cell Biology·K Schulze-OsthoffP H Krammer
Jan 1, 1993·The Journal of Experimental Medicine·E RouvierP Golstein
Feb 25, 1994·Science·L ShiA H Greenberg
Aug 1, 1993·Trends in Biochemical Sciences·M K Collins, A Lopez Rivas

❮ Previous
Next ❯

Citations

Aug 27, 1999·Journal of Cellular Biochemistry·N ShojaeeD Shepro
Oct 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M J Smyth
Apr 16, 1998·Springer Seminars in Immunopathology·J A TrapaniV R Sutton
Oct 31, 1996·Experientia·M F LavinQ Song
Jan 5, 2011·Apoptosis : an International Journal on Programmed Cell Death·Anupriya KhareVineeta Bal
May 5, 2001·Pharmacology & Therapeutics·K SharmaY F Shi
Sep 1, 1996·Research in Immunology·J P Abastado
Apr 27, 2000·Current Opinion in Immunology·J A TrapaniM J Smyth
Jun 24, 1999·Cytokine & Growth Factor Reviews·R HofmeisterS K Durum
Sep 12, 1998·British Journal of Haematology·B D SmithR J Jones
Jun 1, 1996·The Journal of Cell Biology·M D JacobsenM C Raff
Jun 1, 1996·The Journal of Cell Biology·M WeilM C Raff
Apr 1, 1996·The Journal of Experimental Medicine·N ZamzamiG Kroemer
Dec 3, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K SamejimaW C Earnshaw
Feb 3, 1999·Annals of the New York Academy of Sciences·T Ozawa
Feb 28, 2002·Annual Review of Immunology·John H Russell, Timothy J Ley
Apr 29, 1998·Annual Review of Physiology·V J Kidd
Jan 5, 2000·American Journal of Respiratory and Critical Care Medicine·S HashimotoH Nakajima
Jan 1, 2008·Reviews in the Neurosciences·Troy T Rohn, Elizabeth Head
Jul 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrew J BredemeyerTimothy J Ley
Mar 1, 2000·Journal of Neuroimmunology·K A Sabelko-DownesA H Cross
Oct 11, 2005·American Journal of Reproductive Immunology : AJRI·Tatjana Bogovic CrncicDaniel Rukavina
Mar 11, 2011·Scandinavian Journal of Immunology·G LaskarinD Rukavina
Jul 19, 2002·Experimental Eye Research·Arno HueberPeter Johannes Esser
Feb 15, 2000·International Reviews of Immunology·S T JuM Ozdemirli
Jan 5, 2002·Annals of the New York Academy of Sciences·Z SzondyL Fésüs
Apr 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H InoueI Saito
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J H LaiS H Han
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·C M Mueller, D W Scott
Jan 1, 1997·International Reviews of Immunology·G Dennert
Mar 11, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·M J PinkoskiT Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.