The Tat pathway of plant pathogen Dickeya dadantii 3937 contributes to virulence and fitness

FEMS Microbiology Letters
Manuel Rodríguez-SanzLuis Rey

Abstract

Protein secretion plays a very important role in the virulence of the bacterium Dickeya dadantii, the causative agent of soft rot disease, in a wide range of plant species. We studied the contribution of the twin-arginine translocation (Tat) protein system to the adaptation of D. dadantii 3937 to different growth conditions and to the interaction with the plant host. First, a list of 44 putative Tat substrates was obtained using bioinformatic programs taking advantage of the availability of the complete sequence of this bacterium. Second, a tatC mutant strain was constructed and analysed. The mutant displayed a pleiotropic phenotype, showing limited growth in an iron-depleted medium, higher sensitivity to copper, reduced motility on soft agar plates and attenuated virulence in witloof chicory leaves. Our results indicate the Tat system as an important determinant of the virulence and fitness of D. dadantii 3937. Potential Tat substrates related to the tatC mutant phenotype are discussed.

References

Aug 1, 1992·Molecular Microbiology·N Hugouvieux-Cotte-Pattat, J Robert-Baudouy
Jan 1, 1987·Analytical Biochemistry·B Schwyn, J B Neilands
Nov 1, 1996·Molecular Microbiology·B C Berks
Mar 30, 2001·Molecular Plant-microbe Interactions : MPMI·E López-SolanillaP Rodríguez-Palenzuela
Feb 15, 2002·Journal of Bacteriology·Judah L RosnerRobert G Martin
May 30, 2002·Proceedings of the National Academy of Sciences of the United States of America·Urs A OchsnerMichael L Vasil
Feb 4, 2003·Journal of Bacteriology·Kieran DilksMechthild Pohlschröder
Jun 6, 2003·Molecular Microbiology·Bérengère IzeTracy Palmer
Jun 6, 2003·Molecular Microbiology·Stefania MeloniJosé M Palacios
Aug 28, 2003·Molecular Microbiology·Kostas HatzixanthisFrank Sargent
Sep 3, 2004·Molecular Microbiology·Darby G Brown, Caitilyn Allen
Sep 7, 2004·The Journal of Biological Chemistry·Bérengère IzeTracy Palmer
Sep 21, 2004·Proteomics·Nasrin Kazemi-PourNicole Hugouvieux-Cotte-Pattat
Jul 5, 2005·BMC Bioinformatics·Jannick Dyrløv BendtsenSøren Brunak
Mar 15, 2006·Molecular Plant-microbe Interactions : MPMI·Isabelle CaldelariTracy Palmer
Jun 8, 2006·Annual Review of Microbiology·Philip A LeeGeorge Georgiou
Oct 6, 2006·Molecular Microbiology·Anastasias EconomouAnthony P Pugsley
May 1, 2007·Applied and Environmental Microbiology·Enid T GonzálezCaitilyn Allen
Aug 29, 2007·International Journal of Medical Microbiology : IJMM·Emmy De BuckJozef Anné
Jul 23, 2008·Molecular Microbiology·Yann Ferrandez, Guy Condemine
Aug 22, 2008·Trends in Microbiology·Emmy De BuckJozef Anné
Feb 10, 2009·Microbiology·María Antúnez-LamasPablo Rodríguez-Palenzuela
Jan 1, 2003·Molecular Plant Pathology·Ian K TothPaul R J Birch

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