The TEA transcription factor Tec1 confers promoter-specific gene regulation by Ste12-dependent and -independent mechanisms.

Eukaryotic Cell
Barbara HeiseHans-Ulrich Mösch

Abstract

In Saccharomyces cerevisiae, the TEA transcription factor Tec1 is known to regulate target genes together with a second transcription factor, Ste12. Tec1-Ste12 complexes can activate transcription through Tec1 binding sites (TCSs), which can be further combined with Ste12 binding sites (PREs) for cooperative DNA binding. However, previous studies have hinted that Tec1 might regulate transcription also without Ste12. Here, we show that in vivo, physiological amounts of Tec1 are sufficient to stimulate TCS-mediated gene expression and transcription of the FLO11 gene in the absence of Ste12. In vitro, Tec1 is able to bind TCS elements with high affinity and specificity without Ste12. Furthermore, Tec1 contains a C-terminal transcriptional activation domain that confers Ste12-independent activation of TCS-regulated gene expression. On a genome-wide scale, we identified 302 Tec1 target genes that constitute two distinct classes. A first class of 254 genes is regulated by Tec1 in a Ste12-dependent manner and is enriched for genes that are bound by Tec1 and Ste12 in vivo. In contrast, a second class of 48 genes can be regulated by Tec1 independently of Ste12 and is enriched for genes that are bound by the stress transcription factors ...Continue Reading

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Citations

May 18, 2011·Nucleic Acids Research·Stefan BroosPieter De Bleser
Jan 22, 2013·Archives of Insect Biochemistry and Physiology·Grażyna AndruszewskaMarcin Schmidt
Sep 14, 2012·Molecular Microbiology·Hee-Soo ParkJae-Hyuk Yu
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Dec 31, 2019·Molecular Systems Biology·Mario LeutertJudit Villén

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