PMID: 2109088Mar 1, 1990Paper

The TEACL method of DNA-DNA hybridization: technical considerations

Journal of Molecular Evolution
J R Powell, A Caccone

Abstract

This paper emanated from a conference concerning the value, accuracy, and technical considerations of DNA-DNA hybridization for evolutionary studies. Our laboratory has been performing the so-called TEACL (tetraethylammonium chloride) method, and we have amassed sufficient data to indicate that this method is very powerful if performed properly with correct analyses. Here we address five technical considerations: (1) We present empirical data that size correction for tracer length is legitimate and accurate. (2) We show that the error of delta Tm measurement does not significantly increase with increasing distance up to at least 10 degrees C. (3) The error distribution for delta Tm does not deviate from the expected normal distribution indicating parametric statistics are probably legitimate for analyses. (4) Using a known phylogeny we examined the resolving power of the technique by showing that at least five taxa can be correctly placed in phylogenies with a maximum delta Tm of 2.5 degrees C. (5) To date, all our data sets based on DNA-DNA hybridization are very robust with respect to analytical procedures in that every algorithm used on the data sets has yielded identical trees with nearly identical branch lengths. Neverthel...Continue Reading

References

Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J R PowellC Yoon
Jan 1, 1988·Journal of Molecular Evolution·A CacconeJ R Powell
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W B Melchior, P H Von Hippel
Jan 1, 1974·Methods in Enzymology·R J BrittenB R Neufeld
Aug 1, 1970·Quarterly Reviews of Biophysics·D E Kohne
Jan 20, 1967·Science·W M Fitch, E Margoliash
Jan 1, 1970·Biopolymers·F N HayesD L Williams
Jan 1, 1981·Journal of Molecular Evolution·J A HuntR J Britten
Dec 1, 1980·Journal of Molecular Evolution·T J HallR J Britten
Apr 1, 1965·Journal of Molecular Biology·D M CROTHERSB H ZIMM
Jan 1, 1984·Evolution; International Journal of Organic Evolution·Joseph Felsenstein
Nov 1, 1987·Evolution; International Journal of Organic Evolution·Adalgisa Caccone, Jeffrey R Powell
Aug 1, 1989·Evolution; International Journal of Organic Evolution·Adalgisa Caccone, Jeffrey R Powell
Sep 1, 1990·Evolution; International Journal of Organic Evolution·Kathryn GoddardJeffrey R Powell

❮ Previous
Next ❯

Citations

Sep 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R J MacIntyre
Mar 1, 1990·Journal of Molecular Evolution·A Caccone, J R Powell
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·J G Wetmur
Jan 12, 1993·Biochemistry·W A ReesP H von Hippel
Jan 1, 1994·International Journal of Legal Medicine·A MöllerB Brinkmann
May 1, 1992·Journal of Molecular Evolution·M S SpringerR J Britten
Sep 1, 1990·Evolution; International Journal of Organic Evolution·Kathryn GoddardJeffrey R Powell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.