PMID: 19132204Jan 10, 2009Paper

The technique of measuring thrombin generation with fluorogenic substrates: 3. The effects of sample dilution

Thrombosis and Haemostasis
Erik De SmedtH Coen Hemker

Abstract

Assessing the clotting function inevitably brings about dilution of plasma. With the existing techniques of thrombin generation (TG) measurement, dilution ranges from 2:3 to 1:8. However, the possibility that dilution alters procoagulant and anticoagulant pathways differently has not been examined. We investigated the effects of dilution on the thrombin generation process and found that the anticoagulant pathways are far more affected by dilution than the procoagulant pathways. That is, when prothrombin and antithrombin concentrations are kept constant, dilution of plasma does not significantly affect tissue factor (TF)-driven thrombin generation. We demonstrate that dilution of plasma slows down the inhibitory activity of tissue factor pathway inhibitor (TFPI) to a greater extent when compared with the down regulation by diluting procoagulant factors. Dilution of plasma has also a negative effect on the participation of the antihaemophiliac factors VIII and IX in TG driven by contact activation or low TF concentration. We also investigated the effect of dilution on the participation of the anticoagulant system that consists of thrombomodulin, protein C and protein S (APC system). We found that plasma dilution causes a loss of ...Continue Reading

Citations

Oct 14, 2010·PLoS Computational Biology·Manash S ChatterjeeScott L Diamond
Dec 22, 2009·American Journal of Clinical Pathology·H Coenraad HemkerMikhail Roshal
Apr 3, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·N K JhaM V Ovanesov
Aug 20, 2016·Anesthesia and Analgesia·Alexander Y MitrophanovJaques Reifman
Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·K E Brummel-ZiedinsK G Mann
Jul 16, 2010·Haemophilia : the Official Journal of the World Federation of Hemophilia·S C NairA Srivastava
Apr 18, 2014·Thrombosis and Haemostasis·M NinivaggiB de Laat
Jun 6, 2012·Clinical Chemistry·Marisa NinivaggiTheo Lindhout
Nov 19, 2010·Current Medical Research and Opinion·R AltmanG Di Girolamo
Jul 19, 2016·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Kenny M HanssonAnn Lövgren
Apr 14, 2017·Scientific Reports·Elena I SinauridzeFazoil I Ataullakhanov
Feb 29, 2020·Research and Practice in Thrombosis and Haemostasis·Søren Risom KristensenShona Pedersen

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