The telomerase activity and expression of hTERT gene can serve as indicators in the anti-cancer treatment of human ovarian cancer

European Journal of Obstetrics, Gynecology, and Reproductive Biology
Peng-Ming SunJaild Sehouli

Abstract

The aim of this study was to evaluate the clinicopathological significance of telomerase activity and expression of hTERT gene in human ovarian cancer. The potential value of them as indicators for chemotherapy in ovarian cancer cells was also studied. A total of 73 samples and ovarian cancer cell lines HO-8910 and COC1 were studied. Telomerase activity was detected by PCR-TRAP-ELISA assay and the expression of the hTERT mRNA was analyzed by semi-quantitative RT-PCR. Alteration of the telomerase activity and hTERT mRNA were also analyzed in the ovarian cancer cells treated with different concentration and different time of cisplatin. Cytogenetic analysis was performed to compare the telomere status in the OH-8910 cells pre- and post-cisplatin treatment. The associations between these two markers and cisplatin induced-apoptosis were respectively analyzed in COC1 cells by the flow cytometry. Telomerase activity are highly increased in malignancy (0.795+/-0.168(A450-655 nm)) than borderline (0.389+/-0.174(A450-655 nm)), benign tumors (0.236+/-0.102(A450-655 nm)) and normal ovary (0.213+/-0.070(A450-655 nm)) (p < 0.05). Twenty samples showed detectable levels of hTERT. The hTERT gene positive lesion showed significantly higher telo...Continue Reading

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Citations

Jun 7, 2012·Journal of Molecular Modeling·Porika MahendarAbbagani Sadanandam
Nov 19, 2010·Molecular Biology Reports·Aneta WojtylaBlazej Rubis
Apr 19, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Elisabetta KuhnIe-Ming Shih
Oct 12, 2012·The Journal of Biological Chemistry·Ravi KasiappanWenlong Bai
May 31, 2011·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Chi-Hau Chen, Ruey-Jien Chen
Mar 31, 2011·Nature Reviews. Endocrinology·Furio PaciniStefania Marchisotta
Feb 23, 2019·Cells·Taichi Mizushima, Hiroshi Miyamoto
Feb 5, 2008·Cancer Research·Roscoe KlinckSherif Abou Elela
Oct 28, 2019·ACS Omega·Diana C Díaz-CartagenaCarlos R Cabrera

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