The telomerase inhibitor MST-312 synergistically enhances the apoptotic effect of doxorubicin in pre-B acute lymphoblastic leukemia cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Narges GhasemimehrAhmad Fatemi

Abstract

There have been consistent efforts in concomitant administration of chemotherapeutic agents such as doxorubicin beside other more tumor-specific drugs in order to increase sensitivity of tumor cells to the cytotoxicity of doxorubicin and reduce necessary chemotherapeutic dosage. Telomerase-targeted therapy for cancer has received great attention because telomerase is expressed in almost all cancer cells but is inactive in most normal somatic cells. The present study was aimed to investigate the effect of telomerase inhibitor MST-312, a chemically modified derivative of epigallocatechin gallate (EGCG), on doxorubicin-induced apoptosis in pre-B acute lymphoblastic leukemia cells. The pre-B ALL cell lines (NALM-6 and REH) were cultured and treated with MST-312 and doxorubicin, separately and in combination. Cell viability was measured by trypan blue staining and MTT assay. Annexin-V/7-AAD staining by flow cytometry was used for evaluation of apoptosis. Gene expression of hTERT, c-Myc, Bax and Bcl-2, was detected by the quantitative Real-Time PCR. Our results showed that MST-312 exerted dose-dependent short-term cytotoxic and apoptotic effects on pre-B ALL cells. Combination of MST-312 and doxorubicin synergistically enhanced the c...Continue Reading

References

Feb 10, 2004·Cancer Treatment Reviews·Sabine L A PlasschaertEveline S J M de Bont
Oct 30, 2009·Archivum Immunologiae Et Therapiae Experimentalis·Yi-Wei ZhangLei Wei
Nov 5, 2010·Pharmacogenetics and Genomics·Caroline F ThornRuss B Altman
Mar 14, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stephen P HungerWilliam L Carroll
Jun 23, 2012·Cardiovascular Research·Yan ZhangRui-Ping Xiao
Jul 31, 2012·Cancer Treatment Reviews·Maria Ruden, Neelu Puri
Mar 26, 2013·Lancet·Hiroto InabaCharles G Mullighan
Jan 3, 2014·The Journal of Clinical Investigation·Yoshihiko IchikawaHossein Ardehali
Aug 27, 2014·Pharmacogenomics·Elixabet Lopez-LopezAfrica Garcia-Orad
Oct 14, 2014·Molecular Cancer·Resham Lal GurungM Prakash Hande
May 30, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ahmad FatemiAhmad Kazemi
Jun 10, 2016·International Journal of Oncology·Nesstor Pilco-Ferreto, Gloria M Calaf
Sep 23, 2016·European Journal of Medicinal Chemistry·Philip A WaghornKatherine A Vallis

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