The telomere-specific non-LTR retrotransposons SART1 and TRAS1 are suppressed by Piwi subfamily proteins in the silkworm, Bombyx mori.

Cellular & Molecular Biology Letters
T TatsukeTakahiro Kusakabe

Abstract

The telomere structures in Bombyx mori are thought to be maintained mainly by the transposition of the specialized telomeric retroelements SART and TRAS. The silkworm genome has telomeric TTAGG repeats and telomerase, but this telomerase displays little or no activity. Here, we report that the transcription of the telomeric retroelements SART1 and TRAS1 is suppressed by the silkworm Piwi subfamily proteins BmAgo3 and Siwi. The silkworm Piwi subfamily was found to be expressed predominantly in the gonads and early embryo, as in other model organisms, but in BmN4 cultured cells, these proteins formed granules that were separate from the nuage, which is a different behaviour pattern. The expression of TRAS1 was increased in BmN4 cells when BmAgo3 or Siwi were silenced by RNAi. Our results suggest that B. mori Piwi proteins are involved in regulating the transposition of telomeric retroelements, and that the functional piRNA pathway is conserved in BmN4 cultured cells.

References

May 12, 2000·European Journal of Biochemistry·T Sasaki, H Fujiwara
Feb 2, 2002·The EMBO Journal·Hidekazu Takahashi, Haruhiko Fujiwara
Nov 18, 2003·Annual Review of Genetics·Mary-Lou Pardue, P G DeBaryshe
Sep 1, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Haruhiko FujiwaraKenji K Kojima
Nov 2, 2005·Development Genes and Evolution·Hajime NakaoToshio Kanda
Feb 3, 2006·Genes & Development·Mikhail SavitskyVladimir Gvozdev
Feb 16, 2006·Nucleic Acids Research·Haruna TsukiokaTakahiro Kusakabe
Apr 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ai Khim Lim, Toshie Kai
May 26, 2007·Cell·Marianthi KiriakidouZissimos Mourelatos
Sep 20, 2008·Insect Biochemistry and Molecular Biology·Shinpei KawaokaToru Shimada
Jan 24, 2009·Nature Reviews. Molecular Cell Biology·V Narry KimMikiko C Siomi

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Citations

Jan 7, 2016·Frontiers in Microbiology·Ekaterina LazarevaSergey Morozov
Nov 20, 2015·Seminars in Cell & Developmental Biology·Robyn S M Lim, Toshie Kai
Mar 26, 2013·Insect Molecular Biology·L ZhuT Kusakabe
Jul 18, 2015·Insect Biochemistry and Molecular Biology·Li ZhuTakahiro Kusakabe
Apr 21, 2018·International Journal of Molecular Sciences·Muhammad Salman HameedGuang Yang
Aug 11, 2020·PLoS Genetics·Xiaoxi LiuYoichiro Kamatani
Jun 12, 2019·Wiley Interdisciplinary Reviews. RNA·Anna KolliopoulouLuc Swevers
Aug 23, 2019·The Journal of Biological Chemistry·Borja Barbero Barcenilla, Dorothy E Shippen
Dec 22, 2011·Molecular Biology Reports·Zhiqing LiTakahiro Kusakabe
Sep 25, 2017·Biochemical and Biophysical Research Communications·Anandrao Ashok PatilTakahiro Kusakabe
Dec 20, 2011·Insect Biochemistry and Molecular Biology·Isao KobayashiShuichiro Tomita

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