PMID: 2116313Aug 1, 1990Paper

The temporal expression of amyloid precursor protein mRNA in vitro in dissociated hippocampal neuron cultures

Experimental Neurology
M J StrongR M Garruto

Abstract

The subunit protein of the neurofibrillary tangle, the core protein of the neuritic plaque, and the amyloid of the cerebrovasculature in Alzheimer disease and normal aging is a unique 42-amino acid protein (amyloid beta-protein), suggesting a common origin for these pathological entities. However, the expression of the amyloid precursor protein mRNA (APP mRNA) from which the amyloid beta-protein is derived varies between specific neuronal populations. To determine the conditions under which neuronal synthesis of amyloid beta-protein might contribute to the formation of these structures, we have studied the temporal pattern of APP mRNA expression in developing fetal rabbit hippocampal neurons in vitro. Using in situ hybridization with a biotinylated riboprobe transcribed from a cDNA which includes the region encoding the amyloid beta-protein, we have observed a developmentally specific pattern of APP mRNA hybridization during neuronal maturation in vitro.

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Feb 1, 1992·Molecular and Chemical Neuropathology·K C Breen
Jan 11, 1991·Brain Research. Brain Research Reviews·D C GajdusekK K Hsiao
Jul 1, 1997·Brain Research. Molecular Brain Research·H A Rohan de SilvaA J Patel
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Sep 17, 2002·Journal of Neuropathology and Experimental Neurology·Mieczyslaw Marcinkiewicz
Mar 1, 1995·Journal of Neurobiology·O Steward, C S Wallace
Feb 1, 1995·Current Opinion in Neurobiology·O Steward

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