The temporal profile of calcium transients in voltage clamped gastric myocytes from Bufo marinus

The Journal of Physiology
J G McGeownF S Fay

Abstract

1. Decay in intracellular calcium concentration ([Ca2+]i) was recorded following step depolarizations in voltage clamped gastric myocytes from Bufo marinus. 2. Depolarizations (300 ms) to +10 mV were followed by three phases of [Ca2+]i decay with repolarization to both -110 and -50 mV. The decline was initially rapid (mean fractional decay rate = 81 +/- 11%s-1 at -110 mV), then slowed (decay rate = 14 +/- 2%s-1) and finally accelerated again (decay rate = 24 +/- 3%s-1; n = 19). 3. The initial phase of rapid decay became shorter as the length of the depolarizing pulse increased but was unaffected by changes in pulse voltage. 4. The delayed acceleration in [Ca2+]i decay was no longer seen when the duration of the depolarizing pulses was reduced to 100 ms, but was clearly evident following 500 ms pulses. This phase was abolished when the depolarizing voltage was altered to minimize the rise in [Ca2+]i. 5. Ryanodine and caffeine had no effect on the temporal profile of [Ca2+]i decay. 6. Removal of extracellular Na+ decreased the decay rate during all three phases at -110 mV, but this effect was particularly marked for the initial rapid phase of decay, the rate of which was reduced by 75%. A delayed increase in decay rate was still ...Continue Reading

Citations

Apr 28, 2004·Cell Calcium·T B BoltonV Pucovsky
Feb 14, 2002·Biochemical Pharmacology·C Camello-AlmarazP J Camello
Oct 29, 2015·The Journal of Physiology·Calum WilsonJohn G McCarron
May 12, 1998·The Journal of Physiology·M J Taggart, S Wray
Jan 12, 2013·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·John G McCarronSusan Chalmers
Sep 24, 1998·Biophysical Journal·T Kamishima, J G McCarron
Apr 1, 1999·Annual Review of Physiology·T B BoltonD V Gordienko
May 5, 2012·Pflügers Archiv : European journal of physiology·John G McCarronSusan Chalmers
Feb 14, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Calum WilsonJohn G McCarron
May 18, 1999·The American Journal of Physiology·G R Monteith, M P Blaustein

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