The Tenascin-C-Derived Peptide VSWRAPTA Promotes Neuronal Branching Via Transcellular Activation of the Focal Adhesion Kinase (FAK) and the ERK1/2 Signaling Pathway In Vitro

Molecular Neurobiology
Marvin JarockiA Faissner

Abstract

The central nervous system (CNS) of mammals has a limited regeneration capacity after traumatic events, which causes chronic functional disability. The development of biomaterials aims at providing support for the regeneration process. One strategy integrates peptides that mimic functional domains of extracellular matrix (ECM) or cell adhesion molecules with synthetic polymers designed to present growth-supporting cues to the neuronal microenvironment. Thus, small peptide sequences originating from molecules of the ECM may serve as promising bio-additives, acting as artificial matricryptins to gear cellular processes. The glycoprotein tenascin-C (Tnc) is a major constituent of the ECM of the developing brain and persists in the neurogenic regions of the adult CNS. It is a multimodular glycoprotein that comprises distinct domains with neurite growth promoting and axon growth repulsing properties. In the present study, the novel peptide motif VSWRAPTA that is encoded in the neurite growth promoting 6th fibronectin type III repeat close to the alternative splice site of Tnc was tested for its effects on neuron differentiation. When this newly synthesized biomimetic peptide was added to cultures of embryonic cortical neurons it sig...Continue Reading

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Methods Mentioned

BETA
dissection
Assay
PCR
scraping
Protein Assay

Software Mentioned

ImageJ
TotalLab Quant
REST
VAWTSPRA Scram
NeuronJ
SPSS
AxioVision
Neurite Tracer
Light Cycler® 96

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