The Termite Gut Microflora as an Oxygen Sink: Microelectrode Determination of Oxygen and pH Gradients in Guts of Lower and Higher Termites.

Applied and Environmental Microbiology
Andreas BruneJohn A Breznak

Abstract

Clark-type oxygen microelectrodes and glass pH microelectrodes, each with a tip diameter of <=10 (mu)m, were used to obtain high-resolution profiles of oxygen concentrations and pH values in isolated termite guts. Radial oxygen profiles showed that oxygen penetrated into the peripheral hindgut contents up to about 150 to 200 (mu)m below the epithelial surface in both the lower termite Reticulitermes flavipes (Kollar) and the higher termite Nasutitermes lujae (Wasmann). Only the central portions (comprising less than 40% of the total volume) of the microbe-packed, enlarged hindgut compartments ("paunches") were completely anoxic, indicating that some members of the hindgut microbiota constitute a significant oxygen sink. From the slopes of the oxygen gradients, we estimated that the entire paunches (gut tissue plus resident microbiota) of R. flavipes and N. lujae accounted for 21 and 13%, respectively, of the respiratory activity of the intact animals. Axial oxygen profiles also confirmed that in general, only the paunches were anoxic in their centers, whereas midguts and posterior hindgut regions contained significant amounts of oxygen (up to about 50 and 30% air saturation, respectively). A remarkable exception to this was the...Continue Reading

References

May 1, 1978·Applied and Environmental Microbiology·M L EutickM Slaytor
May 1, 1978·Applied and Environmental Microbiology·J E Schultz, J A Breznak
Jan 1, 1982·Annual Review of Microbiology·J A Breznak
Feb 1, 1979·Applied and Environmental Microbiology·D E BignellJ M Anderson
May 1, 1983·Applied and Environmental Microbiology·D A Odelson, J A Breznak
Sep 1, 1991·Journal of Chemical Ecology·G W Felton, S S Duffey

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Citations

Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
May 25, 2006·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Peter Stief, Gundula Eller
Jan 31, 2007·Applied and Environmental Microbiology·Michael PesterAndreas Brune
Jan 1, 2004·FEMS Microbiology Ecology·Andreas KapplerAndreas Brune
Oct 30, 2012·Applied and Environmental Microbiology·Shamayim T Ramírez-PueblaEsperanza Martínez-Romero
May 20, 2015·Archives of Insect Biochemistry and Physiology·Zachary J Karl, Michael E Scharf
Jul 15, 2015·Applied and Environmental Microbiology·Panagiotis SapountzisMichael Poulsen
Dec 15, 2015·Applied and Environmental Microbiology·Aram MikaelyanAndreas Brune
May 1, 2016·Applied and Environmental Microbiology·Ines M HeisterkampPeter Stief
May 19, 2005·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Martin Zimmer, Andreas Brune
Sep 11, 2007·Applied and Environmental Microbiology·John T Wertz, John A Breznak
Oct 6, 2009·Archives of Insect Biochemistry and Physiology·Marsha M WheelerMichael E Scharf
Apr 23, 2011·Journal of Economic Entomology·Paul S Botch, Timothy M Judd
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Scott M GeibMing Tien
Oct 6, 2012·Critical Reviews in Biotechnology·Anas Saadeddin
Jul 13, 2011·PloS One·Michael E ScharfDrion G Boucias
May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
Mar 3, 2011·Cellular and Molecular Life Sciences : CMLS·Yuichi Hongoh
Feb 16, 2013·Nature Reviews. Microbiology·Rachel L Morris, Thomas M Schmidt
Aug 1, 2013·Applied Microbiology and Biotechnology·Helmut KönigJürgen Fröhlich
Jul 15, 2015·Nature Communications·Javier A Ceja-NavarroEoin L Brodie
Jan 19, 2016·Frontiers in Microbiology·Nurdyana Abdul RahmanPhilip Hugenholtz
Oct 6, 2000·Annual Review of Microbiology·H Cypionka
Mar 14, 1998·Proceedings of the National Academy of Sciences of the United States of America·L MargulisS C Lo
Feb 17, 2007·Journal of Basic Microbiology·David Kamanda NgugiHamadi Iddi Boga

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