The tetrasaccharide L-alpha-D-heptose1-->2-L-alpha-D-heptose1--> 3-L-alpha-D-heptose1-->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody.

Infection and Immunity
Silvia BorrelliA A Lindberg

Abstract

A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdo(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep alpha 1-->2Hep alpha 1-->3Hep alpha 1-->Kdo together with part of lipid A, in...Continue Reading

References

Jul 1, 1991·Research in Microbiology·D J MaskellE R Moxon
Nov 1, 1990·Biomedical & Environmental Mass Spectrometry·N J PhillipsJ M Griffiss
Jun 1, 1986·Journal of Bacteriology·P J HitchcockD C Morrison
Mar 15, 1987·Carbohydrate Research·M CarofL Szabó
Jun 1, 1969·European Journal of Biochemistry·C GalanosO Westphal
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Nov 15, 1984·European Journal of Biochemistry·F MichonJ H Banoub
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Aug 1, 1994·Journal of Bacteriology·M CaroffD Karibian
Sep 1, 1993·Anesthesia and Analgesia·W D Hoffman, C Natanson
Mar 1, 1993·Microbiological Reviews·A F VerheulJ T Poolman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.