PMID: 9555911Apr 29, 1998Paper

The tfdK gene product facilitates uptake of 2,4-dichlorophenoxyacetate by Ralstonia eutropha JMP134(pJP4)

Journal of Bacteriology
J H LeveauJan Roelof van der Meer

Abstract

Uptake of 2,4-dichlorophenoxyacetate (2,4-D) by Ralstonia eutropha JMP134(pJP4) was studied and shown to be an energy-dependent process. The uptake system was inducible with 2,4-D and followed saturation kinetics in a concentration range of up to 60 microM, implying the involvement of a protein in the transport process. We identified an open reading frame on plasmid pJP4, which was designated tfdK, whose translation product TfdK was highly hydrophobic and showed resemblance to transport proteins of the major facilitator superfamily. An interruption of the tfdK gene on plasmid pJP4 decimated 2,4-D uptake rates, which implies a role for TfdK in uptake. A tfdA mutant, which was blocked in the first step of 2,4-D metabolism, still took up 2,4-D. A mathematical model describing TfdK as an active transporter at low micromolar concentrations fitted the observed uptake data best.

References

Jan 1, 1985·Journal of Bacteriology·R H Don, J M Pemberton
Jul 7, 1995·The Journal of Biological Chemistry·A E Jessen-MarshallR J Brooker
Oct 1, 1994·Applied and Environmental Microbiology·S TaghaviM Mergeay
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Jan 1, 1997·Applied and Environmental Microbiology·K Filer, A R Harker

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Citations

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Feb 2, 2002·Applied and Environmental Microbiology·Andrew C Hawkins, Caroline S Harwood
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Aug 10, 2005·Canadian Journal of Microbiology·Emma R MasterWilliam W Mohn
Sep 4, 2004·Environmental Microbiology·Jan Roelof van der MeerMarco Jaspers
Oct 20, 1999·Critical Reviews in Biotechnology·B K SinghK K Tripathi
Apr 12, 2001·Letters in Applied Microbiology·C W SmejkalH M Lappin-Scott
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