PMID: 8955303Dec 1, 1996Paper

The tfdR gene product can successfully take over the role of the insertion element-inactivated TfdT protein as a transcriptional activator of the tfdCDEF gene cluster, which encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4)

Journal of Bacteriology
J H Leveau, J R van der Meer

Abstract

The tfdT gene is located upstream of and transcribed divergently from the tfdCDEF chlorocatechol-degradative operon on plasmid pJP4 of Ralstonia eutropha (formerly Alcaligenes eutrophus) JMP134. It is 684 bp long and encodes a 25-kDa protein. On the basis of its predicted amino acid sequence, the TfdT protein could be classified as a LysR-type transcriptional regulator. It has the highest degree of similarity with the proteins TcbR, ClcR, and TfdR, which are involved in the regulation of chloroaromatic breakdown. Despite this homology, the TfdT protein failed to activate the expression of its presumed target operon, tfdCDEF. This failure could be attributed to the inability of TfdT to bind the tfdC promoter region, an absolute requirement for transcriptional activation. Sequence analysis downstream of the tfdT gene revealed the presence of an insertion element-like element. We postulate that this element disrupted the tfdT open reading frame, leading to a premature termination and the production of a truncated, disfunctional TfdT protein. As an alternative to the inactivated TfdT protein, we propose that the product of the tfdR gene (or its identical twin, tfdS), located elsewhere on plasmid pJP4, can successfully take over the...Continue Reading

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Citations

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Jul 28, 2004·Applied Microbiology and Biotechnology·W Park, E L Madsen
Apr 16, 2019·Frontiers in Microbiology·Inna A Suvorova, Mikhail S Gelfand
Jul 11, 2002·Journal of Bacteriology·Glenn R JohnsonJim C Spain
Aug 6, 2004·Applied and Environmental Microbiology·Liz J Shaw, Richard G Burns
May 5, 2005·Applied and Environmental Microbiology·Johan H J Leveau, Steven E Lindow
Feb 8, 2006·Applied and Environmental Microbiology·Jacob BaelumCarsten Suhr Jacobsen
Jul 11, 2002·Journal of Bacteriology·Iris PlumeierDietmar H Pieper
Oct 7, 2004·Applied and Environmental Microbiology·Tina A MüllerHans-Peter E Kohler
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·David Tropel, Jan Roelof van der Meer
Nov 9, 2010·The Journal of Steroid Biochemistry and Molecular Biology·Masae HorinouchiToshiaki Kudo

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