The TFG-TEC fusion gene created by the t(3;9) translocation in human extraskeletal myxoid chondrosarcomas encodes a more potent transcriptional activator than TEC

Carcinogenesis
Bobae LimJungho Kim

Abstract

The t(3;9)(q11-q12;q22) translocation associated with human extraskeletal myxoid chondrosarcomas results in a chimeric molecule in which the N-terminal domain (NTD) of the TFG (TRK-fused gene) is fused to the TEC (Translocated in Extraskeletal Chondrosarcoma) gene. Little is known about the biological function of TFG-TEC. Because the NTDs of TFG-TEC and TEC are structurally different, and the TFG itself is a cytoplasmic protein, the functional consequences of this fusion in extraskeletal myxoid chondrosarcomas were examined. The results showed that the chimeric gene encoded a nuclear protein that bound DNA with the same sequence specificity as the parental TEC protein. Comparison of the transactivation properties of TFG-TEC and TEC indicated that the former has higher transactivation activity for a known target reporter containing TEC-binding sites. Additional reporter assays for TFG (NTD) showed that the TGF (NTD) of TFG-TEC induced a 12-fold increase in the activation of luciferase from a reporter plasmid containing GAL4 binding sites when fused to the DNA-binding domain of GAL4, indicating that the TFG (NTD) of the TFG-TEC protein has intrinsic transcriptional activation properties. Finally, deletion analysis of the function...Continue Reading

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Citations

Apr 10, 2014·International Journal of Molecular Medicine·Margherita CerroneGerardo Botti
Aug 19, 2014·Cancer Genetics·Cory J BroehmThèrése Bocklage
Feb 26, 2016·Breast Cancer Research and Treatment·Maria Vittoria DieciCeline Lefebvre
Jan 25, 2019·The Journal of Biological Chemistry·Kha The NguyenCheol-Sang Hwang
Jan 12, 2021·The Journal of Pathology·Sarmishta KannanKevin B Jones
May 13, 2021·Journal of Cutaneous Pathology·Keisuke GotoArnaud de la Fouchardière

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