PMID: 12755688May 21, 2003Paper

The Theodore Bücher lecture. Investigating signal transduction with genetically encoded fluorescent probes

European Journal of Biochemistry
Tullio PozzanRüdiger Rudolf

Abstract

Ca2+ and cAMP are ubiquitous second messengers in eukaryotes and control numerous physiological responses ranging from fertilization to cell death induction. To distinguish between these different responses, their subtle regulation in time, space and amplitude is needed. Therefore, the characterization of the signalling process requires measurement of second messengers with tools of precise localization, high dynamic range and as little disturbance of cell physiology as possible. Recently, fluorescent proteins of marine jellyfish have given rise to a set of genetically encoded biosensors which fulfil these criteria and which have already led to important new insights into the subcellular handling of Ca2+ and cAMP. The use of these probes in combination with new microscopical methods such as two-photon microscopy now enables researchers to study second messenger signalling in intact tissues. In this review, the genetically encoded measurement probes and their origin are briefly introduced and some recent insights into the spatio-temporal complexity of both Ca2+ and cAMP signalling obtained with these tools are discussed.

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Citations

Mar 17, 2009·Journal of Molecular and Cellular Cardiology·Elinor J Griffiths
Jan 1, 2004·The Journal of Biological Chemistry·María F Cano AbadTullio Pozzan
Jan 13, 2006·British Journal of Pharmacology·Stefan R Nahorski
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Apr 25, 2008·Current Protocols in Immunology·Carl H June, Jonni S Moore
Mar 7, 2020·Frontiers in Cellular and Infection Microbiology·Gustavo BenaimNathalia Martinez-Sotillo
Aug 4, 2005·Cell Calcium·Ole H PetersenAlexei Verkhratsky
Oct 8, 2004·Journal of the American Chemical Society·Jianmin GaoEric T Kool

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