The Theophylline Aptamer: 25 Years as an Important Tool in Cellular Engineering Research

ACS Synthetic Biology
Alexandra WristRyan M Summers

Abstract

The theophylline aptamer was isolated from an oligonucleotide library in 1994. Since that time, the aptamer has found wide utility, particularly in synthetic biology, cellular engineering, and diagnostic applications. The primary application of the theophylline aptamer is in the construction and characterization of synthetic riboswitches for regulation of gene expression. These riboswitches have been used to control cellular motility, regulate carbon metabolism, construct logic gates, screen for mutant enzymes, and control apoptosis. Other applications of the theophylline aptamer in cellular engineering include regulation of RNA interference and genome editing through CRISPR systems. Here we describe the uses of the theophylline aptamer for cellular engineering over the past 25 years. In so doing, we also highlight important synthetic biology applications to control gene expression in a ligand-dependent manner.

Citations

Apr 4, 2021·Life·Huanhuan Ge, Mario Andrea Marchisio
May 6, 2021·Chemical Communications : Chem Comm·Yan LiuLiang Xu
Jun 3, 2021·Talanta·Arghya SettJean-Jacques Toulmé
Jul 10, 2021·World Journal of Microbiology & Biotechnology·Tholasi Nadhan NavienMarimuthu Citartan
Oct 5, 2021·Chemical Society Reviews·Lanlan ChenHuanghao Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

Biomaterial Engineering

Advances in biomaterial engineering have permitted the development of sophisticated drug-releasing materials with a biomimetic 3D support that allow a better control of the microenvironment of transplanted cells. Here is the latest research.

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

Related Papers

Nature Reviews. Urology
Clemens Thoma
Proceedings of the National Academy of Sciences of the United States of America
Lele LiDaniel S Kohane
Annual Review of Biochemistry
Zachary F HallbergMing C Hammond
© 2021 Meta ULC. All rights reserved