The theoretical ratio of immunogold labeling of deplasticized epoxy sections and acrylic sections

Micron : the International Research and Review Journal for Microscopy
S H Brorson, F Skjørten

Abstract

The purpose of this study was to predict the ratio of immunogold labeling of deplasticized epoxy sections and LR-White sections on the basis of theoretical considerations. Tissues used in experiments were pancreas, pituitary, kidney, thyroid, and fibrin. Antigens used as test proteins were glucagon, somatostatin, thyroglobulin, chromogranin A, ACTH (= Adrenocorticotropt hormone), amyloid A, and fibrinogen. These are proteins of different sizes. The quotient labelingdeplasticized/labelingLR-white was deduced theoretically and compared to measurements based on immunogold experiments to obtain a theoretical model with acceptable correlation to the measurements. This study describes a theory--expressed mathematically--for what happens at the molecular level in immunoelectron microscopy at the surface of deplasticized epoxy sections and acrylic sections. The theory explains why we normally get about the same amount of immunogold labeling using LR-White sections (acrylic resin) and deplasticized epoxy sections. Taking the nuances into account, the theory indicates increased usefulness of deplasticized epoxy sections when the diameter of the protein carrying the epitopes decreases.

References

Nov 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Mar, T N Wight
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E KellenbergerM Wurtz
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·S H BrorsonF Skjørten
Dec 1, 1993·Acta histochemica·M Kasper, A Migheli
Jan 1, 1993·Ultrastructural Pathology·M M SilverS S Hearn
Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten
Jun 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten

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Citations

Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Aug 4, 1999·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Dec 26, 2006·Micron : the International Research and Review Journal for Microscopy·Sverre-Henning Brorson, Finn P Reinholt
Dec 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nigel P GoodeAndrew F Mooney

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