The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections

Micron : the International Research and Review Journal for Microscopy
S H Brorson, F Skjørten

Abstract

The purpose of this study was to predict the ratio of immunogold labeling of LR-White sections and epoxy sections using theoretical methods. Tissues used in the experiments were pancreas, pituitary, kidney, thyroid and fibrin. Antigens used as test proteins were glucagon, somatostatin, thyroglobulin, chromogranin A, ACTH (adrenocorticotropt hormone), amyloid A and fibrinogen. These are proteins of different sizes. The quotient labelingLR-White/labelingepoxy was deduced theoretically and compared to calculations based on practical immunogold experiments. The theoretically deduced formula showed acceptable correlation to these calculations. This study gives a theory--expressed mathematically--for what is happening on the molecular level at the surface of resin sections in immunoelectron microscopy. The theory explains why acrylic resins normally are better suited for immunoelectron microscopy than epoxy sections, and indicates increased usefulness of epoxy sections when the diameter of the protein carrying the epitope decreases.

References

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Jan 1, 1995·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten

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Citations

Jun 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson, F Skjørten
Jun 29, 2004·Micron : the International Research and Review Journal for Microscopy·Sverre-Henning Brorson
Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Feb 13, 2001·Micron : the International Research and Review Journal for Microscopy·S H Brorson, G H Nguyen
Apr 27, 2002·Micron : the International Research and Review Journal for Microscopy·S-H Brorson
Oct 1, 1996·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Mar 11, 1999·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Aug 4, 1999·Micron : the International Research and Review Journal for Microscopy·S H Brorson
Nov 2, 1999·Micron : the International Research and Review Journal for Microscopy·S H BrorsonS Rashid
Aug 11, 2000·Micron : the International Research and Review Journal for Microscopy·S H BrorsonI K Woxen
Feb 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·S H BrorsonF Skjørten
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·G Mayer, M Bendayan
Dec 26, 2006·Micron : the International Research and Review Journal for Microscopy·Sverre-Henning Brorson, Finn P Reinholt
Jan 3, 2006·Micron : the International Research and Review Journal for Microscopy·Lene B SolbergFinn P Reinholt
Mar 11, 2000·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·S H Brorson

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