The Therapeutic Effect of ICAM-1-Overexpressing Mesenchymal Stem Cells on Acute Graft-Versus-Host Disease

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Bo TangYi Zhang

Abstract

Mesenchymal stem cells (MSCs) do not readily migrate to appropriate sites, and this creates a major obstacle for their use in the treatment of graft-versus-host disease (GVHD). Intercellular adhesion molecule-1 (ICAM-1) can guide the homing of various immune cells to the proper anatomical location within secondary lymphoid organs (SLOs), which are the major niches for generating immune responses or tolerance. MSCs rarely migrate to SLOs after intravenous infusion, and are constitutively low expression of ICAM-1. So in our previous work, ICAM-1 was engineered into a murine MSC line C3H10T1/2 by retrovirus transfection system (ICAM-1MSCs). Here, we hypothesized that ICAM-1highMSCs may significantly improve their immunomodulatory effect. We used different co-culture methods combined with real-time PCR and flow cytometry to evaluate ICAM-1highMSCs immunomodulatory effect on dendritic cells (DCs) and T cells in vitro and in vivo. MSCs were labeled with carboxyfluorescein diacetate succinimidylester (CFSE) to detect its distribution in mouse model. Our in vitro analyses revealed ICAM-1 MSCs could suppress DCs maturation according to co-culture methods and suppress the T cell immune response according to the mixed lymphocyte response ...Continue Reading

Citations

Jun 27, 2019·Stem Cell Research & Therapy·Nopmanee TaechangamDori L Borjesson
Feb 15, 2020·Journal of Orthopaedic Surgery and Research·Ashim GuptaNicola Maffulli
Mar 25, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ligia Lins de CastroPatricia Rieken Macêdo Rocco
Apr 18, 2020·Stem Cell Research & Therapy·Amandda Évelin Silva-CarvalhoFelipe Saldanha-Araujo
Apr 14, 2020·Cancer Cell International·Xinyi ZhouBaoan Chen
Aug 28, 2020·Frontiers in Immunology·Siyu LiuShu Guo
Mar 20, 2021·Stem Cell Research & Therapy·Alexander MarkovAli Hassanzadeh

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