The therapeutic effect of Ilex pubescens extract on blood stasis model rats according to serum metabolomics

Journal of Ethnopharmacology
Di CaoJing Jin

Abstract

Ilex pubescens Hook. et Arn (MDQ), a traditional Chinese herb, is used in the treatment of cardiovascular diseases. However, the mechanisms underlying the preventive effect of MDQ on blood stasis remain unclear. In this study, serum metabolomics integrated with a biochemical assay strategy were established to evaluate the preventive effect and mechanism of action of MDQ on rats with acute blood stasis. Forty-nine rats were divided into seven groups: the control group, model group, aspirin treatment group (30 mg/kg), clopidogrel treatment group (8 mg/kg) and three MDQ treatment groups (250, 500 and 1000 mg/kg). A hybrid quadrupole time of flight mass spectrometry (QTOF/MS) coupled to ultra-high-performance liquid chromatography (UPLC) was applied for profiling the serum metabolites. The multivariate data analysis techniques using unsupervised principal component analysis (PCA) and supervised orthogonal projections to latent structures discriminant analysis (OPLS-DA) were used for pattern recognition and distinguishing variabilities among groups. MDQ protected the rats against blood stasis, as evidenced by the restoration of the anti-platelet aggregation activity, fibrinogen concentration, prothrombin time, thrombin time, activat...Continue Reading

Citations

Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dongshuai ShenJianyong Li
Dec 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min HaoChunqin Mao
Apr 2, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Yidian JinXue Xiao

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