The thermostability of the RTS,S/AS01 malaria vaccine can be increased by co-lyophilizing RTS,S and AS01.

Malaria Journal
Juliette FortpiedDominique Lemoine

Abstract

Developing thermostable vaccines is a challenge for pharmaceutical companies due to the inherent instability of biological molecules in aqueous solution. The problem is even more stringent in regions subjected to high temperatures in which protective cold chain is difficult to maintain due to a lack of infrastructure. Here, a simple, cost-effective solution to increase the thermostability of the malaria candidate vaccine RTS,S/AS01 is described. This vaccine currently needs to be stored between 2 and 8  °C due to the sensitivity of liquid AS01 to higher temperatures. The strategy was to increase thermostability by co-lyophilizing the RTS,S antigen and AS01. Co-lyophilization was achieved in a solution containing 5% sucrose, 10 mM potassium phosphate and 0.0312% polysorbate 80 at pH 6.1. The physicho-chemical characteristics and immunogenic properties of the resulting solid product, called CL-vac, fresh or stored at high temperature, were compared to those of the candidate RTS,S/AS01. CL-vac proved to be acceptable in terms of visual appearance and physico-chemical characteristics. The structural integrity of both RTS,S and AS01 within CL-vac and its equivalence to the RTS,S/AS01 candidate vaccine were shown. Further, the stabil...Continue Reading

References

Oct 7, 2009·Human Vaccines·Joe CohenAmanda Leach
Jan 3, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mark T OrrThomas S Vedvick
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Citations

Jan 24, 2021·Advanced Drug Delivery Reviews·Kendall B Preston, Theodore W Randolph
Mar 17, 2021·Expert Review of Vaccines·Yizhi Qi, Christopher B Fox

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Methods Mentioned

BETA
transmission electron microscopy
electrophoresis
enzyme-linked immunosorbent assay
dynamic light scattering
ELISA
electron microscopy

Software Mentioned

Soft Max Pro

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Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.