The thermotropic phase behavior of cationic lipids: calorimetric, infrared spectroscopic and X-ray diffraction studies of lipid bilayer membranes composed of 1,2-di-O-myristoyl-3-N,N,N-trimethylaminopropane (DM-TAP)

Biochimica Et Biophysica Acta
R N LewisR N McElhaney

Abstract

The thermotropic phase behavior of lipid bilayer model membranes composed of the cationic lipid 1,2-di-O-myristoyl-3-N,N,N-trimethylaminopropane (DM-TAP) was examined by differential scanning calorimetry, infrared spectroscopy and X-ray diffraction. Aqueous dispersions of this lipid exhibit a highly energetic endothermic transition at 38.4 degrees C upon heating and two exothermic transitions between 20 and 30 degrees C upon cooling. These transitions are accompanied by enthalpy changes that are considerably greater than normally observed with typical gel/liquid--crystalline phase transitions and have been assigned to interconversions between lamellar crystalline and lamellar liquid--crystalline forms of this lipid. Both infrared spectroscopy and X-ray diffraction indicate that the lamellar crystalline phase is a highly ordered, substantially dehydrated structure in which the hydrocarbon chains are essentially immobilized in a distorted orthorhombic subcell. Upon heating to temperatures near 38.4 degrees C, this structure converts to a liquid-crystalline phase in which there is excessive swelling of the aqueous interlamellar spaces owing to charge repulsion between, and undulations of, the positively charged lipid surfaces. The...Continue Reading

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Citations

Jun 11, 2004·Biophysical Journal·Andrey A GurtovenkoIlpo Vattulainen
Mar 22, 2012·Small·Selver AhmedStephanie L Wunder
Mar 4, 2009·Journal of Liposome Research·Sarantis GiatrellisGeorge Nounesis
Aug 4, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Gary C H Mo, Christopher M Yip
Jun 19, 2009·The Journal of Physical Chemistry. B·Markus S MiettinenMikko Karttunen

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