The three-dimensional high resolution structure of human interferon alpha-2a determined by heteronuclear NMR spectroscopy in solution

Journal of Molecular Biology
W KlausH Senn

Abstract

The solution structure of recombinant human interferon alpha-2a (Roferon-A) has been determined by multidimensional heteronuclear NMR spectroscopy. The calculations using simulated annealing produced a family of 24 convergent structures which satisfy the experimental restraints comprising 1541 NOE-derived inter-proton distances, 187 dihedral restraints, 66 pairs of hydrogen bond restraints, and six upper and lower limits for two disulfide bridges. The fractional labeling of methyl groups allowed their direct and unambiguous stereospecific assignment which proved to be essential for obtaining a high resolution of the structures. A best fit superposition of residues 10 to 47, 50 to 101 and 111 to 157 gives an rms deviation of 0.62 A for the backbone heavy atoms and 1.39 A for all heavy atoms of these segments. The dominant feature of the structure is a cluster of five alpha-helices, four of which are arranged to form a left-handed helix bundle with an up-up-down-down topology and two over-hand connections. The interpretation of heteronuclear 15N-¿1H¿ NOE data shows the co-existence of flexible regions within an otherwise rigid framework of the protein. Four stretches of pronounced flexibility can be located: Cys1-Ser8, Gly44-Ala5...Continue Reading

Citations

Jan 18, 2012·Immunology and Cell Biology·Philippa HillyerRonald L Rabin
Nov 16, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Valentina SalucciFabio Palombo
Dec 4, 2003·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Dimitris Platis, Graham R Foster
Nov 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K E MogensenG Uzé
Oct 27, 2007·Antioxidants & Redox Signaling·Jui-Yoa Chang
Jun 1, 2006·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Caroline Sommereyns, Thomas Michiels
Jan 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Hana SchmeisserKathryn C Zoon
Jan 21, 2009·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Vaheh OganesyanWilliam F Dall'Acqua
Sep 24, 1999·Protein Science : a Publication of the Protein Society·R M ArduiniD P Baker
Mar 23, 2004·Annual Review of Immunology·Sidney PestkaPaul B Fisher
Mar 3, 2012·The AAPS Journal·Caroline Watson, Joshua S Sharp
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·L C RoismanG Schreiber
Nov 18, 2004·Immunological Reviews·Sidney PestkaMark R Walter
Nov 6, 2013·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Anna MicallefJean Calleja-Agius
Sep 29, 2004·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Tim D JonesMatthew P Baker
May 3, 2014·Preparative Biochemistry & Biotechnology·Nawal Abd El-Baky, Elrashdy M Redwan
Apr 27, 2001·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J B Barreto FilhoR R Golgher
May 6, 2014·Protein Expression and Purification·Regina L BisKrishna M G Mallela
Nov 20, 2016·The Journal of Pharmacy and Pharmacology·Brittney J Mills, Jennifer S Laurence Chadwick
Aug 3, 1999·Nature Biotechnology·C C ChangP A Patten
Mar 27, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jing LiWuyuan Lu
Sep 27, 2006·Protein Science : a Publication of the Protein Society·Sabine R Quadt-AkabayovJacob Anglister
Aug 19, 2017·Journal of Drug Targeting·Annabelle Herrington-SymesSteve Brocchini
Aug 17, 2006·The Journal of Clinical Endocrinology and Metabolism·Peter M van KoetsveldLeo J Hofland
Feb 6, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Hyong-Ha KimNam Woong Song
May 23, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jyothi KumaranEleanor N Fish
May 1, 2008·The Journal of Pharmacology and Experimental Therapeutics·Lasse Dissing-OlesenBente Finsen
Sep 6, 2000·European Journal of Biochemistry·V C BlankL P Roguin
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R HuK Zoon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

© 2021 Meta ULC. All rights reserved