The three-dimensional organization of polytene nuclei in male Drosophila melanogaster with compound XY or ring X chromosomes.

Genetics
D Mathog, J Sedat

Abstract

The three-dimensional organization of polytene chromosomes within nuclei containing rearranged X chromosomes was examined in male Drosophila melanogaster. Salivary glands of third instar larvae containing either an inverted X chromosome (YSX.YL, In(1)EN/O) or a ring X chromosome (R(1) 2/BSYy+) were fixed, embedded, and serially sectioned. The nuclei in contiguous groups of cells were modeled and analyzed. We find that for both genotypes the three-dimensional behavior at each euchromatic locus is independent of the orientation of the chromosome on which it resides, independent of the behavior of loci not closely linked to it, and not similar in neighboring cells. The preference for right-handed chromosome coiling noted in previous studies is shown to be independent of homologous pairing. However, a relation between the extent of chromosome curvature and the handedness of chromosome coiling is present only in homologously paired chromosomes. The attached-XY chromosome has two previously undescribed behaviors: a nearly invariant association of the euchromatic side of the proximal heterochromatin/euchromatin junction with the nucleolus and a frequent failure of this site to attach to the chromocenter. The relative chromosome arm po...Continue Reading

Citations

Feb 26, 2000·Annual Review of Genetics·D Zickler, N Kleckner
Feb 1, 1991·Genetic Analysis, Techniques and Applications·P LichterD C Ward
Feb 1, 2007·DNA and Cell Biology·Gaspar BanfalviAlexei G Basnakian
Dec 14, 1990·Science·L Manuelidis
Aug 1, 2006·Nature Genetics·Helen PickersgillBas van Steensel
Apr 26, 2008·FEBS Letters·Bernike KalverdaMaarten Fornerod

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