PMID: 1486007Mar 1, 1992Paper

The three-dimensional structure of frozen-hydrated bacteriophage phi X174

Journal of Structural Biology
N H OlsonN L Incardona

Abstract

The three-dimensional structure of bacteriophage phi X174 (phi X174) was determined to approximately 2.6 nm resolution from images of frozen-hydrated 114 S particles. The outer surface of phi X174 is characterized by several prominent features: (i) 12 mushroom-shaped caps (approximately 7.1 nm wide x 3.8 nm high) are situated at each of the vertices of the icosahedral virion and extend to a maximum radius of 16.8 nm; (ii) a "collar" of density surrounds the base of each apical cap; and (iii) 20 conical protrusions (approximately 2.3 nm high) lie along the three-fold symmetry axes. The caps have a pentagonal morphology composed of five globular "subunits" and appear to be loosely connected to the underlying capsid. The distribution of the four gene products present in virions (60 copies each of gpF, gpG, and gpJ, and 12 copies of gpH), and the single-stranded DNA (ssDNA) genome cannot be directly discerned in the reconstructed density map, although plausible assignments can be made on the basis of solvent-excluded volume estimates and previous biochemical data. Thus, gpG accounts for most of the mass in the caps; gpH, a presumed cap protein, cannot be identified in part due to the symmetry-averaging procedures, but may be partia...Continue Reading

References

Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T S BakerM Bina
Apr 5, 1985·Journal of Molecular Biology·J Lepault, K Leonard
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·A B Burgess
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Citations

Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T J SmithT S Baker
May 1, 1999·Journal of Structural Biology·D M BelnapT S Baker
Nov 14, 1997·Journal of Molecular Biology·I S GabashviliP Serwer
May 18, 1999·Journal of Molecular Biology·T DoklandM G Rossmann
Sep 7, 2014·Applied and Environmental Microbiology·Simon J LabrieSylvain Moineau

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