The thrombin binding aptamer GGTTGGTGTGGTTGG forms a bimolecular guanine tetraplex

Biochemical and Biophysical Research Communications
Markéta FialováMichaela Vorlícková

Abstract

In the literature, the thrombin binding aptamer GGTTGGTGTGGTTGG is generally taken as a prototype of an intramolecular guanine tetraplex of DNA. Our results, however, show that this notion is not true in aqueous solutions. This conclusion is based on a dependence of the CD spectra on aptamer concentration, migration of the aptamer in polyacrylamide gels, and the Ferguson analysis of the gel migration data. The presented data document that the aptamer forms a bimolecular tetraplex. We furthermore show that only an extension of the aptamer by a sequence containing further guanines, or an elongation of loop regions, causes that its tetraplex folding is intramolecular.

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Citations

Jun 20, 2008·The Journal of Physical Chemistry. B·Ji Wook Shim, Li-Qun Gu
Jul 28, 2010·Nucleic Acids Research·Aurore GuédinJean-Louis Mergny
Dec 18, 2012·International Journal of Molecular Sciences·Elsa Rouah-MartinJohan Robbens
Jan 2, 2007·Biochemical and Biophysical Research Communications·Iva KejnovskáMichaela Vorlícková
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Mar 1, 2012·Chemistry : a European Journal·Aurore De RacheClaudine Buess-Herman
Dec 6, 2014·Angewandte Chemie·Radislav A PotyrailoMilos Todorovic
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Feb 3, 2019·Sensors·Nerea De AchaFrancisco J Arregui
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