PMID: 9529384May 16, 1998Paper

The thrombospondin receptor CD47 (IAP) modulates and associates with alpha2 beta1 integrin in vascular smooth muscle cells

Molecular Biology of the Cell
X Q Wang, W A Frazier

Abstract

The carboxyl-terminal domain of thrombospondin-1 enhances the migration and proliferation of smooth muscle cells. Integrin-associated protein (IAP or CD47) is a receptor for the thrombospondin-1 carboxyl-terminal cell-binding domain and binds the agonist peptide 4N1K (kRFYVVMWKk) from this domain. 4N1K peptide stimulates chemotaxis of both human and rat aortic smooth muscle cells on gelatin-coated filters. The migration on gelatin is specifically blocked by monoclonal antibodies against IAP and a beta1 integrin, rather than alphav beta3 as found previously for 4N1K-stimulated chemotaxis of endothelial cells on gelatin. Both human and rat smooth muscle cells displayed a weak migratory response to soluble type I collagen; however, the presence of 4N1K peptide or intact thrombospondin-1 provoked a synergistic chemotactic response that was partially blocked by antibodies to alpha2 and beta1 integrin subunits and to IAP. A combination of antialpha2 and IAP monoclonal antibodies completely blocked chemotaxis. RGD peptide and antialphav beta3 mAb were without effect. 4N1K and thrombospondin-1 did not augment the chemotactic response of smooth muscle cells to fibronectin, vitronectin, or collagenase-digested type I collagen. Complex fo...Continue Reading

References

Feb 14, 1992·Biochemical and Biophysical Research Communications·A S AschR L Nachman
Jan 1, 1990·Journal of Cardiovascular Pharmacology·T Scott-BurdenF R Bühler
Apr 1, 1987·The Journal of Clinical Investigation·A S AschR L Nachman
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A MajackP Bornstein
Jan 1, 1987·Methods in Enzymology·S A Santoro, W A Frazier
Oct 1, 1994·Current Opinion in Cell Biology·S J ShattilJ S Brugge
Oct 1, 1993·Current Opinion in Cell Biology·B Felding-Habermann, D A Cheresh
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·D CooperM A Vadas
Feb 1, 1994·The Journal of Clinical Investigation·G H van ZantenJ J Sixma
Jan 5, 1996·The Journal of Biological Chemistry·A G GaoW A Frazier
Jul 1, 1996·Journal of Vascular Surgery·P R NelsonK C Kent
Oct 1, 1996·The Journal of Cell Biology·A G GaoW A Frazier
Jun 6, 1997·The Journal of Biological Chemistry·J ChungW A Frazier
Aug 11, 1997·The Journal of Cell Biology·D W DawsonN P Bouck

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Citations

Dec 31, 2002·Journal of Molecular and Cellular Cardiology·Rüdiger BlindtAnja-Katrin Bosserhoff
Apr 20, 2004·The International Journal of Biochemistry & Cell Biology·Olga I SteninaEdward F Plow
Mar 24, 2004·Critical Reviews in Oncology/hematology·Georges BellonArnaud Robinet
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
May 19, 2000·Experimental Gerontology·M J Barnes, R W Farndale
Dec 5, 2000·Matrix Biology : Journal of the International Society for Matrix Biology·H ChenJ Lawler
Sep 9, 2000·Current Opinion in Cell Biology·J Lawler
Nov 18, 1998·Current Opinion in Cell Biology·M E Hemler
Oct 6, 1999·Current Opinion in Cell Biology·L V Parise
Apr 18, 2001·Trends in Cell Biology·E J Brown, W A Frazier
Nov 24, 2001·Trends in Cell Biology·M A Schwartz
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Stephen B WillinghamIrving L Weissman
Nov 1, 2001·Annual Review of Cell and Developmental Biology·J C Adams
Dec 13, 2000·Journal of Molecular and Cellular Cardiology·R BlindtJ vom Dahl
Mar 12, 2009·Cancer Research·Ozge UluçkanKatherine N Weilbaecher
Jul 26, 2011·Cellular and Molecular Life Sciences : CMLS·Olivier Gires
Oct 30, 2012·Expert Opinion on Therapeutic Targets·David R Soto-PantojaDavid D Roberts
Apr 29, 2014·Frontiers in Physiology·Natasha M RogersJeffrey S Isenberg
Oct 19, 1999·Microscopy Research and Technique·T TsakiridisG P Downey
Sep 16, 2006·Human Gene Therapy·Kirsten A Weigel-KelleyArun Srivastava
Jul 17, 2001·Microscopy Research and Technique·M E Bamberger, G E Landreth
Mar 6, 2008·Vascular and Endovascular Surgery·Xiu-Jie WangVivian Gahtan
Aug 7, 2004·The Journal of Biological Chemistry·Tadahiro NumakawaHiroshi Kunugi
Jan 30, 2004·The Journal of Biological Chemistry·Wen-Teng Chang, A-Min Huang
Oct 8, 2014·Developmental Neurobiology·Chung-Pin HsiehA-Min Huang
Feb 13, 2007·Circulation Research·Jeff S IsenbergDavid D Roberts
Aug 13, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Takuya IchiiYoshiki Nishizawa
Sep 1, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Rute MouraMarc F Hoylaerts
Jun 16, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Olga I SteninaEdward F Plow
Jan 12, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Jeff S IsenbergWilliam A Frazier

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